S100B antibody

Catalog No. ABIN1108929

The Mouse Monoclonal anti-S100B antibody (Clone SA-12) (ABIN1108929) specifically detects S100B in WB, IF and IP. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for S100B antibody (ABIN1108929)

Target: S100B (S100 Calcium Binding Protein B (S100B))

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This S100B antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: SA-12

Product Details anti-S100B Antibody

Specificity: This antibody recognizes S100B.

Purification: Affinity chromatography

Immunogen: Bovine brain S-100b.

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1.2 % Sodium Acetate or neutral PBS

Concentration: 0.1 mg/mL

Buffer: 1.2 % Sodium Acetate with 2 mg BSA and 0.01 mg Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Prior to reconstitution store at -20 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for S100B

Target: S100B (S100 Calcium Binding Protein B (S100B))

Alternative Name: S100B

Background: S100 calcium binding protein B or S100beta is a protein of the S-100 protein family. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21, however, this gene is located at 21q22.3. S100B is a glial-derived protein that is a well-established biomarker for severity of neurological injury and prognosis for recovery.1 S100 beta is a calcium-binding protein that is expressed at high levels in brain primarily by astrocytes. Addition of the disulfide-bonded dimeric form of S100 beta to primary neuronal and glial cultures and established cell lines induces axonal extension and alterations in astrocyte proliferation and phenotype, but evidence that S100 beta exerts the same effects in vivo has not been presented. Reeves et al. (1994) demonstrated that the same effects of the S100B protein are exerted in vivo. They found that both astrocytosis and neurite proliferation occurred in transgenic mice expressing elevated levels of S100b. They suggested that these transgenic mice represent a useful model for studies of the role of S100B in glial-neuronal interactions in normal development and function of the brain and for analyzing the significance of elevated levels of the protein in Down syndrome and Alzheimer disease.Synonyms: Astrocyte Marker, Protein S100-B, S-100 protein beta chain, S-100 protein subunit beta, S100 beta, S100 calcium-binding protein B

Gene ID: 525716

NCBI Accession: NP_001029727

UniProt: P02638

Pathways: Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Toll-Like Receptors Cascades, Regulation of long-term Neuronal Synaptic Plasticity, S100 Proteins