SPTAN1 antibody

Catalog No. ABIN1109119

This Mouse Monoclonal antibody specifically detects SPTAN1 in WB, IHC (fro) and EIA. It exhibits reactivity toward Human.

Quick Overview for SPTAN1 antibody (ABIN1109119)

Target: SPTAN1 (Spectrin alpha Chain, Brain (SPTAN1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SPTAN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: AA6

Product Details anti-SPTAN1 Antibody

Specificity: The antibody clone AA6 is specific to the 240 kDa Alpha-Fodrin molecule of all Mammalian nonerythroid cells and Chicken Alpha-Spectrin.

Cross-Reactivity (Details): Species reactivity (tested):Human, Rat, Chicken, Rabbit and Guinea Pig.

Purification: Ig Fraction

Immunogen: Chicken red blood cell membranes purified by hypotonic lysis and mechanical enucleation. Hybridoma produced by fusion between myeloma cells and Balb/c spleen cells.

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, 0.09 % Sodium Azide, 1.0 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for SPTAN1

Target: SPTAN1 (Spectrin alpha Chain, Brain (SPTAN1))

Alternative Name: Spectrin alpha Chain (Brain) / SPTAN1

Background: Fodrin is a universally expressed membrane associated cytoskeletal protein consisting of alpha and beta subunits. The protein is important for maintaining normal membrane structure and supporting cell surface protein function. Alpha Fodrin is one of the important targets cleaved by caspases during apoptosis. The full length 240 kDa protein can be cleaved at several sites within its sequence by activated caspases generating N terminal 150 kDa, C terminal 120 kDa, and 35 kDa major products. Cleavage of alpha Fodrin leads to membrane malfunction and cell shrinkage.Synonyms: Alpha-II spectrin, Fodrin alpha chain, SPTA2, Spectrin non-erythroid alpha chain

Gene ID: 374234

NCBI Accession: NP_001036003

UniProt: P07751

Pathways: Caspase Cascade in Apoptosis, Regulation of Actin Filament Polymerization