Topoisomerase II alpha antibody (N-Term)

Catalog No. ABIN1109282

The Mouse Monoclonal anti-Topoisomerase II alpha antibody is suitable to detect Topoisomerase II alpha in samples from Human, Mouse and Rat. It has been validated for WB.

Quick Overview for Topoisomerase II alpha antibody (N-Term) (ABIN1109282)

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Topoisomerase II alpha antibody is un-conjugated

Application: Western Blotting (WB)

Clone: AK5

Product Details anti-Topoisomerase II alpha Antibody

Binding Specificity: N-Term

Specificity: This antibody reacts with Topo IIa and ß. The antibody detects 170 KD of human Topo IIa and 180 KD of human Topo IIß on Western blot with whole cell lysate.

Cross-Reactivity (Details): Species reactivity (tested):Human (Jurkat, Raji), Mouse (NIH3T3, P19, WR19L), Rat (PC12)

Purification: Protein-A Sepharose

Immunogen: Recombinant N-terminal Topoisomerase II fusion protein

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 50 % glycerol, pH 7.2, without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store (in aliquots) at -20 °C.

Target Details for Topoisomerase II alpha

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Alternative Name: TOP2A

Background: Eukaryotic DNA Topoisomerase II is a ubiquitous ATP-dependent type II Topoisomerase that play an important role in DNA replication, transcription and recombination by changing the degree of superhelicity, catenation and knotting of topologically closed DNA molecules. Two isoforms of DNA Topoisomerases II have been reported in mammalian cells, Topoisomerase IIa and IIß. cDNA sequence of those isoforms reveals that both type II Topoisomerases are structurally and catalytically close. However, Topoisomerase IIß is expressed with no cell cycle dependence, whereas the expression of Topoisomerase IIa is cell cycle-regulated, peaking in G2-M phase and declining to a minimal level at the end of M phase. Topoisomerase IIa is poly-ubiquitinated and degraded preferentially in the apoptosis-induced human epithermoid carcinoma-derived cell line, MA1, in an ATP-and-ubiquitin-dependent manner. Recent studies also indicated that this isoform is selectively cleaved during Fas-mediated T cell apoptosis and Fas-, Staurosporine- and Anisomycin-mediated Jurkat cell apoptosis.Synonyms: DNA topoisomerase 2-alpha, DNA topoisomerase 2-beta, DNA topoisomerase II alpha isozyme, DNA topoisomerase II beta isozyme, TOP2, Top-2

Gene ID: 7153

NCBI Accession: NP_001058

UniProt: P11388

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases