C5 antibody

Catalog No. ABIN111066

The Mouse Monoclonal anti-C5 antibody (Clone HCC5b-1 (neo)) (ABIN111066) specifically detects C5 in WB, IHC (fro) and EIA. The antibody is reactive with Human, Pig, Cow, Goat and Primate samples.

Quick Overview for C5 antibody (ABIN111066)

Target: C5 (Complement Component 5 (C5))

Reactivity: Human, Pig, Cow, Goat, Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This C5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: HCC5b-1 (neo)

Product Details anti-C5 Antibody

Specificity: HCC 5b.1 (neo) allows detection of C5b-9 complexes via activated C5, especially in different forms of glomerulonephritis. Does not react with native C5! Polypeptide Reacting: Mr 200 000 polypeptide of SDS denaturated complement component C5 and C5b of Mr 185 000, after C5b activation, an Mr 60 000 cleavage product also reacts (weakly). Disorders Specifically Detected: Detection of C5b-9 complexes via activated C5, whereas native C5 reacts with antibody HCC 5.1.

Cross-Reactivity (Details): Species reactivity (tested):Human, Primates, Bovine, Pig, Goat.

Purification: Affinity Chromatography on Protein A

Immunogen: Activated Human Complement Component C5

Isotype: IgG1

Application Details

Application Notes: ELISA. Immunoblotting (Western blot). Cytological material. Immunohistochemistry on Frozen Sections (1/10). Incubation time: 1h at RT.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1 mL distilled water.

Handling Advice: This product is photosensitive and should be protected from light

Storage: 4 °C

Storage Comment: Prior to and following reconstitution store the antibody undiluted at 2-8 °C. DO NOT FREEZE!

Target Details for C5

Target: C5 (Complement Component 5 (C5))

Alternative Name: Complement C5

Background: C5 is synthesised in the liver as a single polypeptide chain. Before secretion the molecule is glycosylated and secreted into plasma as a 190 kDa glycoprotein consisting of a disulphide linked alpha-chain (111 kDa) and beta-chain (75 kDa). C5 precursor is first processed by the removal of 4 basic residues, forming two chains, beta and alpha, linked by a disulfide bond. C5 convertase activates C5 by cleaving the alpha chain, releasing C5a anaphylatoxin and generating C5b (beta chain + alpha' chain).Synonyms: CPAMD4, Complement Component 5

Gene ID: 727

NCBI Accession: NP_001726

UniProt: P01031

Pathways: Complement System, Carbohydrate Homeostasis