IGF1R antibody (Chain alpha) (PE)

Catalog No. ABIN111142

Product Details anti-IGF1R Antibody (PE)

Target: IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))

Binding Specificity: Chain alpha

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IGF1R antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Specificity: BM2226R recognises human CD221, a 155 kDa receptor tyrosine kinase, also known as Insulin-like growth factor I receptor (IGF-I Receptor). CD221 is composed of two extracellular alpha-subunits and two transmembrane beta-subunits. Clone 1H7 recognises an epitope in the alpha subunits of CD221, demonstrated by Western blotting (1). CD221 is expressed in a range of tissues, including kidney, liver, placenta, mammary gland, brain, ovary and skin. The ligands for CD221 include IGF-I and IGF-II, which bind to CD221 and activate tyrosine kinase activity, resulting in phosphorylation of several intracellular signalling proteins. Clone 1H7 is reported to partically block binding of IGF-I and IGF-II to CD221 (1). We recommend the use of BM2226LE for this purpose.

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Affinity chromatography on Protein G

Clone: 1H7

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: use 10 μL of neat antibody to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Reconstitute with 1 mL distilled water

Concentration: 0.1 mg/mL

Buffer: Phosphate buffered saline pH 7.4 containing 0.09 % Sodium Azide, 1 % Bovine Serum Albumin

Preservative: Sodium azide

Precaution of Use: Do Not Use Sodium Azide as Preservative.

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.

References for anti-IGF1R antibody (PE) (ABIN111142)

Hantel, Lewrick, Schneider, Zwermann, Perren, Reincke, Süss, Beuschlein: "Anti insulin-like growth factor I receptor immunoliposomes: a single formulation combining two anticancer treatments with enhanced therapeutic efficiency." in: The Journal of clinical endocrinology and metabolism, Vol. 95, Issue 2, pp. 943-52, (2010) (PubMed).

Target Details for IGF1R

Target: IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))

Alternative Name: CD221 / IGF1R (IGF1R Products)

Background: There are two kinds of IGF receptors based on their relative affinities for IGF1 versus IGF2. The IGF1 receptor prefers IGF1 over IGF2 and weakly binds insulin. IGF1 receptor is a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha (130 kD) and two beta (95 kD) subunits. Both the alpha and beta subunits are encoded within a single receptor precursor cDNA. The IGF1 receptor is therefore similar in structure to the insulin receptor. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. The IGF1 receptor is highly expressed in all cell types and tissues and is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival.Synonyms: IGF-I receptor, IGF1 Receptor, Insulin-like growth factor 1 receptor

Gene ID: 3480

NCBI Accession: NP_000866

UniProt: P08069

Pathways: RTK Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Autophagy