F12 antibody (Light Chain)

Catalog No. ABIN111207

Product Details anti-F12 Antibody

Target: F12 (Coagulation Factor XII (F12))

Binding Specificity: Light Chain

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This F12 antibody is un-conjugated

Application: Enzyme Immunoassay (EIA), Functional Studies (Func)

Specificity: Reacts with the light chain of human Factor XII and at 100nM inhibits 50 % of Factor XII activity.

Purification: Protein G affinity chromatography

Immunogen: Purified human Factor XII

Clone: 802

Isotype: IgG1

Application Details

Application Notes: ELISA, to detect/quantitate Factor XII and in coagulation assays.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.95 mg/mL

Buffer: PBS, pH 7.4, without preservative

Preservative: Without preservative

Handling Advice: Aliquot to Avoid multiple freeze-thaw cycles.

Storage: -20 °C

Storage Comment: Upon receipt, store at -20 °C to -70 °C.

Target Details for F12

Target: F12 (Coagulation Factor XII (F12))

Abstract: F12 Products

Synonyms: HAE3 antibody, HAEX antibody, HAF antibody, FXII antibody, coagulation factor XII antibody, coagulation factor XII (Hageman factor) antibody, F12 antibody

Background: Coagulation factor XII circulates in blood as a zymogen. This single chain zymogen is converted to a two chain serine protease with an heavy chain (alpha factor XIIa) and a light chain. The heavy chain contains two fibronectin type domains, two epidermal growth factor (EGF) like domains, a kringle domain and a proline rich domain, whereas the light chain contains only a catalytic domain. On activation, further cleavages takes place in the heavy chain, resulting in the production of beta factor XIIa light chain and the alpha factor XIIa light chain becomes beta factor XIIa heavy chain. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha factor XIIa and then to beta factor XIIa. The active factor XIIa participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. It activates coagulation factors VII and XI. Defects in Factor XII gene do not cause any clinical symptoms and the sole effect is that whole blood clotting time is prolonged.Synonyms: HAF, Hageman factor

Gene ID: 2161

NCBI Accession: NP_000496

UniProt: P00748

Pathways: Complement System