MAP2 antibody (Biotin)

Catalog No. ABIN111292

This Mouse Monoclonal antibody specifically detects MAP2 in WB, IF, IHC (p) and IHC (fro). It exhibits reactivity toward Human, Mouse, Rat, Cow, Chicken, Quail and Xenopus laevis.

Quick Overview for MAP2 antibody (Biotin) (ABIN111292)

Target: MAP2 (Microtubule-Associated Protein 2 (MAP2))

Reactivity: Human, Mouse, Rat, Cow, Chicken, Quail, Xenopus laevis

Host: Mouse

Clonality: Monoclonal

Conjugate: This MAP2 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: AP20

Product Details anti-MAP2 Antibody (Biotin)

Specificity: Anti-MAP-2 recognizes (Mr 300 kDa)MAP-2 protein from bovine brain. The antibody strongly cross-reacts with MAP-2 from human, rat, mouse, Xenopus, quail and chicken brains. Anti-MAP-2 (Clone AP20) reacts with the high molecular weight forms (2a & 2b) of MAP-2 but not with the low molecular weight form (2c). Anti-MAP-2 reacts with dendrites and cell bodies of neurons.

Purification: Protein G chromatography

Immunogen: Bovine brain microtubule associated protein 2.

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry on frozen sections: 1/500. Immunofluorescence: 1/500. WesternBlotting: 1-2 μg/mL for 2hrs at RT). Immunohistology (Formalin/paraffin): 1-2 μg/mL for 30 min at RT. [Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10 mMcitrate buffer, pH 6.0, for 10-20 min. followed by cooling at RT for 20 min. ]Anti-MAP-2 may be used in studies of neuron structure in both normal and malignant braintissue.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: 0.01 M phosphate buffered saline (PBS) pH 7.2, containing, 1.0 % BSA and 0.09 % sodium azide as a preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2-8 °C for one month or (in small aliquots) at -20 °C for longer.

Target Details for MAP2

Target: MAP2 (Microtubule-Associated Protein 2 (MAP2))

Alternative Name: MAP-2

Background: Microtubule Associated Protein 2 exists in two high molecular weight forms (2a and 2b) and a low molecular weight form (2c). The expression of MAP2 is developmentally regulated and its multiple forms arise by alternative splicing of a single gene.Synonyms: MAP2, Microtubule-associated protein 2, Neuronal Marker

Gene ID: 4133

NCBI Accession: NP_002365

UniProt: P11137