Anti-MAP-2 recognizes (Mr 300 kDa)MAP-2 protein from bovine brain and dendrites and cell bodies of neurons. Anti-MAP-2 (Clone AP20) reacts with the high molecular weight forms (2a & 2b) of MAP-2 but not with the low molecular weight form (2c).
Characteristics
Absorption / Emission: 492 nm / 518 nm Molar Ratio: F / P = 3.75
MAP2
Reactivity: Rat
WB, IHC, ICC, IP
Host: Rabbit
Polyclonal
unconjugated
Application Notes
This antibody can be used in immunohistochemistry on tissues or cell usingimmunofluorescence techniques. The suggested working dilution forimmunohistochemical staining is approximately 1: 500. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.1 mg/mL (OD280)
Buffer
0.01 M PBS, pH 7.2, containing 2 mM EDTA, 1 % BSA and 0.09 % sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody at 2-8 °C for one month or (in small aliquots) at -20 °C for longer.
Microtubule Associated Protein 2 exists in two high molecular weight forms (2a and 2b) and a low molecular weight form (2c). The expression of MAP2 is developmentally regulated and its multiple forms arise by alternative splicing of a single gene.Synonyms: MAP2, Microtubule-associated protein 2, Neuronal Marker