CRH antibody

Catalog No. ABIN111806

The Mouse Monoclonal anti-CRH antibody is suitable to detect CRH in samples from Human. It has been validated for IHC (p) and EIA.

Quick Overview for CRH antibody (ABIN111806)

Target: CRH (Corticotropin Releasing Hormone (CRH))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CRH antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Clone: 5D5

Product Details anti-CRH Antibody

Specificity: Recognises a different epitope than clone 4H9 (BM357). 5D5 mAb binding decreases only partly after CA treatment. It is possible that the antigen determinant is exposed more openly after DEP modification of histidine residues, whereas this determinant becomes barely accesible after lysine modification. Human serum, containing CRF-binding protein, inhibits the binding of CRF to this antibody.Aff. Const.:1.5 x 10-8M for native CRF, 2 x 10-9M for modified CRF

Purification: Affinity Chromatography on Protein G

Immunogen: Synthetic Human Corticotropin Releasing Factor

Isotype: IgG1

Application Details

Application Notes: ELISA: High binding constant allows this antibody to be used in a sandwich ELISA withclone number 4H9 (BM357), Sensitivity: 25 pg/mL. Immunohistochemistry on frozen sections: A 4 μg/mL solution has worked well on paraffinsections, after microwave pre-treatment.0.1 % trypsin digestion may also be necessary,The detection system used was avidin-biotin. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CRH

Target: CRH (Corticotropin Releasing Hormone (CRH))

Alternative Name: Corticoliberin

Background: CRF precursor is a 41-amino acid peptide derived from a 196-amino acid preprohormone. CRF is a neuroendocrine hormone secreted by the paraventricular nucleus (PVN) of the hypothalamus and is a key player in the hypothalamic pituitary adrenal axis. It is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed in the placenta. Neuronal CRF is important in a variety of diverse roles including, memory, depression, stress, kidney function, parturition, metabolism and feeding (to mention a few).Synonyms: CRF, CRH, Corticotropin-Releasing Factor, Corticotropin-Releasing Hormone

Gene ID: 1392

NCBI Accession: NP_000747

UniProt: P06850

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Activity, Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Feeding Behaviour