This antibody X-6 is useful for the detection of the Fibronectin Receptor, or CD49e. Detects Human granulocytes, monocytes and platelets. Cross reactivity: unknown. X-6 detects an antigen of 154-180 kDa by Immunoprecipitation. Antigen distribution on Isolated cells and Tissue sections: Granulocytes, monocytes, positive on blood monocyte-derived macrophages days 0-28. Dull staining with 95 % cord blood CD34+ cells, weak reaction on resting neutrophils. Staining increases with maturation of granulocytes. Treatment with fMLP, TNF, GM-CSF and temperature causes positive shift with granulocytes and monocytes. PMA treatment of granulocytes leads to a decrease in the number of positive cells. Positive with cell lines U937, DH39 & MEP, HL-60, NB4, HMC-1. X-6 is positive on lining cells in different infiltrations and endothelial cells in the synovial membrane of RA patients. The staining pattern is similar to CD34 in these patients.
Cross-Reactivity (Details)
Species reactivity (tested):Human: Granulocytes, Monocytes, Platelets.
ITGA5
Reactivity: Human
WB, IF
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Flow Cytometry: use 10 μL stock solution to label 10^6 cells. Immunohistochemistry on Frozen Sections: 4 μg/mL (1/100). Suggested Positive Control: Human tonsil. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Restore with 0.5 mL distilled water
Concentration
0.4 mg/mL
Buffer
Stock solution contains PBS, pH 7.2 with 0.01 % Thiomerosal as preservative and 10 mg/mL BSA as stabilizer.
Handling Advice
Dilute only prior to immediate use
Storage
4 °C
Storage Comment
Store the antibody at 2-8 °C as an undiluted liquid. DO NOT FREEZE! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
The X-6 antibody is useful for the detection of the fibronectin receptor, or CD49e. CD49e is a component of an adhesion receptor heterodimer (α5β1) to fibronectin and invasin. α5β1 recognizes an Arg-Gly-Asp sequence in fibronectin. Binding of fibronectin through α5β1 is important for cell survival and induces apoptosis, particularly in hemopoietic cells.Synonyms: CD49 antigen-like family member E, FNRA, Fibronectin receptor subunit alpha, Integrin alpha-5, Integrin alpha-F, VLA-5, VLA5