Endothelial Cells antibody

Catalog No. ABIN111945

The Rat Monoclonal anti-Endothelial Cells antibody (Clone 1F10) (ABIN111945) specifically detects Endothelial Cells in IHC (fro). The antibody is reactive with Human samples.

Quick Overview for Endothelial Cells antibody (ABIN111945)

Target: Endothelial Cells

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This Endothelial Cells antibody is un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 1F10

Product Details anti-Endothelial Cells Antibody

Purification: Affinity A chromatography.

Immunogen: Cultured HUVECs.

Isotype: IgG2a

Application Details

Application Notes: Immunohistochemistry on Frozen sections: 2 μg/mL (1: 100),Suggested positive control: Human placenta. Antigen Distribution: The antigen is found in variable amounts on freshly isolated isolated HUVE cells. Antigenexpression on cultured HUVEC is dependent on the culture conditions. Best results wereobtained (40-70 % pos. ) by daily renewal of FCS containing medium supplemented withendothelial cell conditioned supernatant whereas commercial available specificendothelial cell media have no influence on the antigen expression (see Table 1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.5 mL distilled water.

Concentration: 0.2 mg/mL

Buffer: PBS, pH 7.2 containing 10 mg/mL bovine serum albumin (BSA) as a stabilizer and 0.01 % thimerosal as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Dilute only prior to immediate use

Storage: 4 °C

Storage Comment: Store the antibody at 2-8 °C as an undiluted liquid. DO NOT FREEZE! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Target Details for Endothelial Cells

Target: Endothelial Cells

Background: Synonyms: Endothelial Cell marker