Endothelial Cells antibody (Biotin)

Catalog No. ABIN5541247

The Biotin-conjugated Rat Monoclonal anti-Endothelial Cells antibody (Clone 1F10) (ABIN5541247) specifically detects Endothelial Cells in IHC (fro). The antibody is reactive with Human samples.

Quick Overview for Endothelial Cells antibody (Biotin) (ABIN5541247)

Target: Endothelial Cells

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This Endothelial Cells antibody is conjugated to Biotin

Application: Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 1F10

Product Details anti-Endothelial Cells Antibody (Biotin)

Specificity: This antibody recognizes an antigen on human continuous blood endothelial cells which are fully differentiated. The antibody staining is restricted to endothelial cells and does not sta in any other cell in tissues or in peripheral blood. On sinusoidal endothelial cells of the liver or lymphatic organs the antigen is inconsistently expressed or absent.

Immunogen: Cultured HUVECs

Isotype: IgG2a

Application Details

Application Notes: Immunohistochemistry on Frozen sections: 2 μg/mL (1:200). Positive control: Human placenta. Does not react on routinely processed paraffin sections.

Restrictions: For Research Use only

Handling

Buffer: 0.01 % thimerosal Stabilizers: 10 mg/mL bovine serum albumin

Storage: 4 °C,-20 °C

Storage Comment: Store lyophilized at 2-8°C. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Endothelial Cells

Target: Endothelial Cells

Background: The antigen is found in variable numbers on freshly isolated umbilical vein endotheli al cells (HUVECs). Antigen expression on cultured HUVECs is dependent on the culture conditions. Best results were obtained (40 - 70 % pos.) by daily renewal of FCS containing medium supplemented with endothelial cell conditioned supernatant whereas commercia lly available endothelial cell media apparently have no influence on the antigen expression.