CD4 antibody
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- Target See all CD4 Antibodies
- CD4
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Reactivity
- Rat
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CD4 antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)
- Purification
- Affinity chromatography.
- Immunogen
- Lymphoblasts.
- Clone
- Rib 5-2
- Isotype
- IgG2a
- Top Product
- Discover our top product CD4 Primary Antibody
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anti-CD4 (CD4) (AA 385-457) antibody
CD4 Reactivity: Human, Mouse, Rat, Chicken, Pig FACS, WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated
anti-CD4 (CD4) antibodyCD4 Reactivity: Human, Mouse, Rat, Chicken, Monkey, Dog, Pig, Cat, Cow, Rabbit, Sheep, Guinea Pig, Goat, Horse, Avian, Donkey, Hamster WB Host: Goat Polyclonal unconjugated
anti-CD4 (CD4) (AA 231-330) antibodyCD4 Reactivity: Human, Mouse, Rat FACS, WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated
anti-CD4 (CD4) (N-Term) antibodyCD4 Reactivity: Human FACS, WB, CyTOF Host: Mouse Monoclonal MEM-241 unconjugated
anti-CD4 (CD4) antibodyCD4 Reactivity: Mouse FACS, IP, IHC (fro), ICC, Func Host: Rat Monoclonal GK1-5 unconjugated
anti-CD4 (CD4) (AA 301-400) antibodyCD4 Reactivity: Human, Mouse, Rat FACS, WB, IHC (p) Host: Rabbit Polyclonal unconjugated
anti-CD4 (CD4) (N-Term) antibody (FITC)CD4 Reactivity: Human FACS Host: Mouse Monoclonal MEM-241 FITC
anti-CD4 (CD4) (N-Term) antibody (PE)CD4 Reactivity: Human FACS Host: Mouse Monoclonal MEM-241 PE
anti-CD4 (CD4) antibody (PE)CD4 Reactivity: Rat FACS Host: Mouse Monoclonal OX-35 PE
anti-CD4 (CD4) (AA 26-200) antibodyCD4 Reactivity: Human WB, IF Host: Rabbit Polyclonal unconjugated
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- Application Notes
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Suitable for Immunohistochemistry (IHC), has been described to work in FACS (see Table1). Recommended Dilutions for IHC: Frozen sections: 1.5 μg/mL (1/200)Paraffin sections: does not react on routinely processed paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Protocol
- Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG (H+L)minimal-cross reaction to rat) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer's hemalum
- Restrictions
- For Research Use only
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- Reconstitution
- Restore with 0.5 mL distilled water.
- Concentration
- 0.3 mg/mL
- Buffer
- PBS, pH 7.2 containing 0.01 % Thiomerosal as preservative and 10 mg/mL BSA as stabilizer.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody at 2-8 °C for one month or at -20 °C for longer.
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- Target
- CD4
- Alternative Name
- CD4 (CD4 Products)
- Background
- CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes ("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.Synonyms: T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
- Gene ID
- 10116
- UniProt
- P05540
- Pathways
- TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events
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