Iba1 antibody (Biotin)

Catalog No. ABIN112010

The Mouse Monoclonal anti-Iba1 antibody is suitable to detect Iba1 in samples from Human and Rat. It has been validated for IHC (p).

Quick Overview for Iba1 antibody (Biotin) (ABIN112010)

Target: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Iba1 antibody is conjugated to Biotin

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 1022-5

Product Details anti-Iba1 Antibody (Biotin)

Specificity: Recognizes AIF-1. This antibody BM4114B reacts with activated Macrophages / Microglial cells.

Purification: Affinity Chromatography.

Immunogen: Purified Human recombinant AIF-1 Epitope is not further characterized.

Isotype: IgG2b

Application Details

Application Notes: Immunohistochemistry on Paraffin Sections: 5 μg/mL (1/40). Pretreatment is not necessary. Suggested Positive Control: Human spleen. This antibody does not react on routinely processed Frozen Sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore by adding 0.5 mL distilled water.

Concentration: 0.2 mg/mL

Buffer: PBS containing 5 mg/mL BSA as a stabilizer and 0.09 % Sodium Azide as a preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: This product is photosensitive and should be protected from light

Storage: 4 °C

Storage Comment: Store the antibody undiluted Prior to and following reconstitution at 2-8 °C. DO NOT FREEZE!

Target Details for Iba1

Target: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Alternative Name: AIF1

Background: Allograft Inflammatory Factor-1 is a Ca2+-binding peptide produced by activated macrophages and microglial cells. It has been suggested that AIF-1 expression is associated with chronic inflammatory processes. AIF-1, like the Ca2+-binding peptides MRP8 and MRP14, is expressed by activated macrophages and might participate in a variety of pathogenic processes in the mammalian brain and in chronic transplant rejection. It has been shown to be expressed early and persistently in chronically rejecting cardiac allografts but not in cardiac syngrafts and host hearts. The recently described apoptosis-inducing factor AIF is not related to AIF-1.Synonyms: AIF-1, Allograft inflammatory factor 1, IBA1, Ionized Calcium-Binding Adapter Molecule 1, Protein G1

Gene ID: 199

NCBI Accession: NP_001614

UniProt: P55008

Pathways: Smooth Muscle Cell Migration