Lamin A/C antibody

Catalog No. ABIN112042

This Mouse Monoclonal antibody specifically detects Lamin A/C in WB, IF, IHC (p), FACS and IHC (fro). It exhibits reactivity toward Human, Mouse, Rat, Cow and Dog.

Quick Overview for Lamin A/C antibody (ABIN112042)

Target: Lamin A/C (LMNA)

Reactivity: Human, Mouse, Rat, Cow, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This Lamin A/C antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 133A2

Product Details anti-Lamin A/C Antibody

Specificity: The antibody 133A2 recognizes an epitope located between residues 598-611 of Lamin A, therefore it reacts exclusively with Lamin A.

Purification: Purified

Immunogen: Partially purified recombinant Human Lamin A.

Isotype: IgG3

Application Details

Application Notes: Immunocytochemistry. Immunohistochemistry on frozen sections (1: 100 - 1: 200 with avidin-biotinylatedhorseradish peroxidase complex (ABC) as detection reagent). Immunoblotting (1: 100 - 1: 1000). Flow cytometry (1: 100 - 1: 200).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: Immunofluorescence protocol - Formaldehyde fixation1. Collect cells from T. c. unit and remove media from petri dish using suction. 2. Wash with 1x PBS and remove. 3. Incubate cells in pre-warm (37°C) Para-Formaldehyde for 12 minutes at roomtemperature on an orbital shaker. 4. Remove PFA and incubate in 0. 5% Triton X-IOO in 1x PBS for 5 minutes at roomtemperature. 5. Prepare blocking reagent, this is also the antibody diluent. 6. Wash cells 2x with 1x PBS at room temperature, for 4 minutes/wash on an orbital shaker. 7. Block with 1 % NCS and 1x PBS for 30 minutes at room temperature. 8. Prepare primary antibodies (50μl/coverslip) and moist staining chambers. 9. Wash cells 2x with lx PBS at room temperature and air dry briefly.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Lamin A/C

Target: Lamin A/C (LMNA)

Alternative Name: Lamin-A/C (LMNA)

Background: Nuclear lamins form a network of intermediate-type filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2. Recent evidence has revealed that mutations in A-type lamins give rise to a range of rare but dominant genetic disorders, including Emery-Dreifuss muscular dystrophy, dilated cardiomyopathy with conduction-system disease and Dunnigan-type familial partial lipodystrophy. In addition, the expression of A-type lamins coincides with cell differentiation and as A-type lamins specifically interact with chromatin, a role in the regulation of differential gene expression has been suggested for A-type lamins.Synonyms: 70 kDa Lamin, LMN1, LMNA, Lamin A, Lamin A + C, Lamin-A/C, NY-REN-32, NYREN32, Nuclear Envelope Marker, Renal carcinoma antigen NY-REN-32

Molecular Weight: 74 kDa (Predicted)

Gene ID: 4000

NCBI Accession: NP_005563

UniProt: P02545

Pathways: Apoptosis, Caspase Cascade in Apoptosis, ER-Nucleus Signaling, Protein targeting to Nucleus