Lamin A/C antibody

Catalog No. ABIN112346

The Mouse Monoclonal anti-Lamin A/C antibody has been validated for WB, IF, FACS and IHC (fro). It is suitable to detect Lamin A/C in samples from Human, Mouse, Rat, Cow, Dog, Hamster and Sheep.

Quick Overview for Lamin A/C antibody (ABIN112346)

Target: Lamin A/C (LMNA)

Reactivity: Human, Mouse, Rat, Cow, Dog, Hamster, Sheep

Host: Mouse

Clonality: Monoclonal

Conjugate: This Lamin A/C antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 131C3

Product Details anti-Lamin A/C Antibody

Specificity: 131C3 reacts with an epitope located between residues 319-566 in Lamin A and C. Immunoblotting of 131C3 recognizes Nuclear Lamins A and C in Human Fibroblasts.

Purification: Purified

Immunogen: Purified Rat liver Lamins.

Isotype: IgG1

Application Details

Application Notes: Immunoblotting: 1/100-1/1000. Flow Cytometry: 1/100-1/200. Immunocytochemistry. Immunohistochemistry on Frozen Sections: 1/100-1/200 with avidin-biotinylatedhorseradish peroxidase complex (ABC) as detection reagent.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Lamin A/C

Target: Lamin A/C (LMNA)

Alternative Name: Lamin-A/C (LMNA)

Background: Nuclear lamins form a network of intermediate-type filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2. Recent evidence has revealed that mutations in A-type lamins give rise to a range of rare but dominant genetic disorders, including Emery-Dreifuss muscular dystrophy, dilated cardiomyopathy with conduction-system disease and Dunnigan-type familial partial lipodystrophy. In addition, the expression of A-type lamins coincides with cell differentiation and as A-type lamins specifically interact with chromatin, a role in the regulation of differential gene expression has been suggested for A-type lamins.Synonyms: 70 kDa Lamin, LMN1, LMNA, Lamin A, Lamin A + C, Lamin-A/C, NY-REN-32, NYREN32, Nuclear Envelope Marker, Renal carcinoma antigen NY-REN-32

Gene ID: 4000

NCBI Accession: NP_005563

UniProt: P02545

Pathways: Apoptosis, Caspase Cascade in Apoptosis, ER-Nucleus Signaling, Protein targeting to Nucleus