Cytokeratin 5 antibody

Catalog No. ABIN112348

Product Details anti-Cytokeratin 5 Antibody

Target: Cytokeratin 5 (KRT5) (Keratin 5 (KRT5))

Reactivity: Human, Mouse, Rat, Pig, Dog, Hamster, Rabbit, Cat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytokeratin 5 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: RCK102 is a Cytokeratin antibody reacting with Cytokeratin 5 and Cytokeratin 8. This monoclonal antibody recognizes virtually all epithelial tissues and carcinomas.

Cross-Reactivity (Details): Also reacts to: KRT8

Purification: Purified

Immunogen: Cytokeratins from a human lung cancer cell line (MR21).

Clone: RCK102

Isotype: IgG1

Application Details

Application Notes: RCK102 is suitable for Immunoblotting, Immunocytochemistry, Immunohistochemistry onfrozen sections and paraffin-embedded tissues and Flow cytometry. Recommended dilutions: 1/100-1/200 for Flow cytometry, and for Immunohistochemistrywith avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and1/100-1/1000 for Immunoblotting applications.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the original vial at 2-8 °C. After reconstitution store undiluted (in aliquots) at -20 °C.

References for anti-Cytokeratin 5 antibody (ABIN112348)

Choi, Lee, Kim, Eom, Jeong, Lee, Park, Jeong, Kwon: "Hyaluronic Acid Coating on Hydrophobic Tracheal Scaffold Enhances Mesenchymal Stem Cell Adhesion and Tracheal Regeneration." in: Tissue engineering and regenerative medicine, Vol. 18, Issue 2, pp. 225-233, (2021) (PubMed).

Target Details for Cytokeratin 5

Target: Cytokeratin 5 (KRT5) (Keratin 5 (KRT5))

Alternative Name: Cytokeratin 5 (KRT5 Products)

Synonyms: 3300001P10Rik antibody, AW146334 antibody, CK5 antibody, K5 antibody, Krt2-5 antibody, Tfip8 antibody, DDD antibody, DDD1 antibody, EBS2 antibody, KRT5A antibody, Sak57 antibody, ckii antibody, wu:fb02h09 antibody, wu:fj02d02 antibody, wu:fk78b12 antibody, zfCKII antibody, KRT75 antibody, CK-5 antibody, Keratin-5 antibody, keratin 5 antibody, Krt5 antibody, KRT5 antibody, krt5 antibody

Background: Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9-7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.Synonyms: 58 kDa Cytokeratin, CK5, Cytokeratin-5, K5, KRT5, Keratin 5, Keratin type II cytoskeletal 5, Keratin-5

Gene ID: 3852

NCBI Accession: NP_000415

UniProt: P13647