NEFM antibody

Catalog No. ABIN112357

This Mouse Monoclonal antibody specifically detects NEFM in WB, IF, IHC (p) and IHC (fro). It exhibits reactivity toward Human, Rat, Monkey, Hamster and Xenopus laevis.

Quick Overview for NEFM antibody (ABIN112357)

Target: NEFM (Neurofilament Triplet M Protein (NEFM))

Reactivity: Human, Rat, Monkey, Hamster, Xenopus laevis

Host: Mouse

Clonality: Monoclonal

Conjugate: This NEFM antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: RNF403

Product Details anti-NEFM Antibody

Specificity: RNF403 reacts exclusively with the phosphorylated isoform of the 160 kDa neurofilament protein.

Purification: Purified

Immunogen: Neurofilament preparation of calf brain tissue.

Isotype: IgG1

Application Details

Application Notes: Immunoblotting. Immunocytochemistry. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin-Embedded Tissues. Recommended Dilutions: 1/50-1/100 for Immunohistochemistry with avidin-biotinylated horseradish peroxidasecomplex (ABC) as detection reagent.1/100-1/500 for Immunoblotting applications.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for NEFM

Target: NEFM (Neurofilament Triplet M Protein (NEFM))

Alternative Name: Neurofilament M (160 kD)

Background: Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. The neurofilament (NF) triplet proteins (70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are normally restricted to neurons. The 70 kDa NF-protein can self-assemble into a filamentous structure, whereas the 160 kDa and 200 kDa NF-proteins require the presence of the 70 kDa NF-protein to co-assemble. All three NF proteins can be detected by immunohistochemical methods at day 9 or 10 after gestation in the mouse embryo. Although IFPs of the neurofilament type are normally restricted to neurons, there are reports on their expression in non-neuronal cells as well. For example, in heart conduction myocytes NF proteins are expressed together with desmin. In tumorpathology ganglioneuroblastomas and some of the other neuroblastomas are strongly positive with the neurofilament antisera. Also, some neuro-endocrine malignancies may show NF positivity. In cell cultures of neural tissues the neurofilament antibodies can monitor in vitro differentiation.Synonyms: (Neuronal Marker), NEF3, NEFM, NF-M, Neurofilament 3, Neurofilament medium polypeptide

Gene ID: 4741

NCBI Accession: NP_005373

UniProt: P07197

Pathways: Brown Fat Cell Differentiation