ITGB1 antibody (C-Term, N-Term)

Catalog No. ABIN112375

This Mouse Monoclonal antibody specifically detects ITGB1 in WB, IF and IHC (fro). It exhibits reactivity toward Human, Mouse and Pig.

Quick Overview for ITGB1 antibody (C-Term, N-Term) (ABIN112375)

Target: ITGB1 (Integrin beta 1 (ITGB1))

Reactivity: Human, Mouse, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This ITGB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 1G2

Product Details anti-ITGB1 Antibody

Binding Specificity: C-Term, N-Term

Purification: Purified

Immunogen: 1G2 is a mouse monoclonal IgG2a, k antibody derived by fusion of SP2/0 mouse myeloma cells with spleen cells from a mouse immunized with a synthetic peptide corresponding to the C-terminal 24 amino acids of integrin b1D including an appending N-terminal cysteine (CQENPIYKS-PINNFKNPNYGRKAGL) coupled to keyhole limpet hemocyanin.

Isotype: IgG2a

Application Details

Application Notes: Suitable for Immunoblotting, Immunocytochemistry, Immunohistochemistry on frozensections. Recommended dilutions: 1/50-1/100 for Flow cytometry, and for Immunohistochemistrywith avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and1/100-1/500 for Immunoblotting applications.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS with 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store lyophilized antibody at -20 °C to -70 °C. Reconstituted antibody should be stored in working aliquots at -20 °C.

Target Details for ITGB1

Target: ITGB1 (Integrin beta 1 (ITGB1))

Alternative Name: CD29 / Integrin beta-1

Background: Integrins are a family of heterodimeric membrane glycoproteins consisting of non-covalently associated a and b subunits. More than 18 a and 8 b subunits with numerous splice variant isoforms have been identified in mammals. In general, integrins function as receptors for extracellular matrix proteins. Certain integrins can also bind to soluble ligands or to counter-receptors on adjacent cells, such as the intracellular adhesion molecules (ICAMs), resulting in aggregation of cells. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis. There are two major forms of integrin b1: b1A and b1D, which differ in 13 amino acids. Their distribution pattern in adult tissues is mutually exclusive. b1A is present in all tissues, except cardiac and skeletal muscle, which instead express the b1D variant.Synonyms: FNRB, Fibronectin receptor subunit beta, ITGB1, Integrin VLA-4 subunit beta, MDF2, MSK12

Gene ID: 9606

UniProt: P05556

Pathways: Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor, Integrin Complex, SARS-CoV-2 Protein Interactome