M13 Bacteriophage G8P Capsid antibody

Catalog No. ABIN112403

Product Details

Target: M13 Bacteriophage G8P Capsid

Reactivity: Bacteriophage M13

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)

Specificity: RL-ph2 reacts with the M13 filamentous phage coat protein g8p with a Molecular Weight of 5 kDa.

Purification: Affinity Chromatography.

Immunogen: Isolated M13 phage coat proteins. Remarks: Derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse.

Clone: RL-ph2

Isotype: IgG2a

Application Details

Application Notes: RL-ph2 is particularly useful for immunoblotting of separated phage proteins, and is alsosuitable for detection of M13 phages in multistep immunoblotting procedures,immunocytochemistry, flow cytometry, affinity chromatography and ELISA. Optimalantibody dilution should be determined by titration, recommended range is 1: 25 - 1: 200 forflow cytometry, and for immunohistochemistry with avidin-biotinylated horseradishperoxidase complex (ABC) as detection reagent, and 1: 100 - 1: 1000 for immunoblottingapplications.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store lyophilized at 2-8 °C. After reconstitution aliquot and store at -20 °C.

Target Details

Target: M13 Bacteriophage G8P Capsid

Target Type: Phage Protein

Background: The display of repertoires of antibody fragments on the surface of filamentous phage offers a new way to produce immunoreagents with defined specificities. Phage derived antibody fragments offer a number of advantages over mouse monoclonal antibodies, such as better clearance from the blood, the possibility to select from human combinatorial libraries and the relative ease by which such fragments can be manipulated. The phage display technique thus facilitates the selection of antibody fragments of therapeutic value or research interest. Antibodies to M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface.Synonyms: Coat protein B, Gene 8 protein, Major coat protein

Gene ID: 927333

NCBI Accession: NP_510890

UniProt: P69541