M13 Bacteriophage G8P Capsid antibody (Biotin)

Catalog No. ABIN125965

Product Details

Target: M13 Bacteriophage G8P Capsid

Reactivity: Bacteriophage M13

Host: Mouse

Clonality: Monoclonal

Conjugate: Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: B62-FE2 binds to an epitope on pVIII (phage coat protein) covering the N-terminal region of g8p AEGDDPAKAAFDSLQASAT (cf. Kneissel et al.)

Purification: Affinity Chromatography on Protein A

Immunogen: fd phages from E. coli F+ strain (JM109)

Clone: B62-FE2

Isotype: IgG2b

Application Details

Application Notes: Phage Display: Immunoassays for the identification of recombinant antigen- orantibody-phages. Detection limit: 10e7 phage particles. Working dilution for ELISA: 1/5,000-1/10,000. Immunoblotting.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, pH 7.4, 0.09 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C up to one month or (in aliquots) at -20 °C for longer.

Target Details

Target: M13 Bacteriophage G8P Capsid

Target Type: Phage Protein

Background: M13 is a filamentous bacteriophage composed of circular single stranded DNA (ssDNA) which is 6407 nucleotides long encapsulated in approximately 2700 copies of the major coat protein P8, and capped with 5 copies of two different minor coat proteins (P9, P6, P3) on the ends. The minor coat protein P3 attaches to the receptor at the tip of the F pilus of the host Escherichia coli. Infection with filamentous phages is not lethal, however the infection causes turbid plaques in E. coli. It is a non-lytic virus. However a decrease in the rate of cell growth is seen in the infected cells. Antibodies to M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface. The display of repertoires of antibody fragments on the surface of filamentous phage offers a new way to produce immunoreagents with defined specificities. Phage derived antibody fragments offer a number of advantages over mouse monoclonal antibodies, such as better clearance from the blood, the possibility to select from human combinatorial libraries and the relative ease by which such fragments can be manipulated. The phage display technique thus facilitates the selection of antibody fragments of therapeutic value or research interest.Synonyms: Coat protein B, Gene 8 protein, Major coat protein

Gene ID: 927333

NCBI Accession: NP_510890

UniProt: P69541