MFGE8 antibody

Catalog No. ABIN112407

The Mouse Monoclonal anti-MFGE8 antibody (Clone MFG-06) (ABIN112407) specifically detects MFGE8 in WB, IHC (p), IF, IHC (fro) and EIA. The antibody is reactive with Human samples.

Quick Overview for MFGE8 antibody (ABIN112407)

Target: MFGE8 (Milk Fat Globule-EGF Factor 8 Protein (MFGE8))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MFGE8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: MFG-06

Product Details anti-MFGE8 Antibody

Specificity: The MFG-06 monoclonal antibody reacts with 40-45 kDa glycoprotein in Human milk fat globule membrane preparation.

Purification: Protein A-affinity chromatography

Purity: > 95 % by SDS-PAGE

Immunogen: Human milk fat globulin membrane preparation

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry on methanol/acetone fixed cultured cells and on cryostat orformalin fixed paraffin-embedded sections (strongly positive on human epithelia-basedstructures). ELISA. Western Blot. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH ~7.4, 15 mM Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for MFGE8

Target: MFGE8 (Milk Fat Globule-EGF Factor 8 Protein (MFGE8))

Alternative Name: Lactadherin

Background: Human Milk Fat Globule 1 (MFG1) is a membrane protein present on normal human breast epithelial cells and cell lines derived from breast carcinomas. Human MFG1 is also localised on the surface of the human milk fat globule. HMFG1 is a differentiation marker and specific breast epithelial marker in normal and neoplastic mammary development. MFG1 Binds specifically to rotavirus and inhibits its replication.Synonyms: Breast epithelial antigen BA46, HMFG, MFGE8, MFGM, Milk fat globule-EGF factor 8

Gene ID: 4240

NCBI Accession: NP_001108086

UniProt: Q08431

Pathways: SARS-CoV-2 Protein Interactome