Vaccinia Virus antibody
VACV
Reactivity: Vaccinia Virus (VACV)
EIA, IHC (p)
Host: Rabbit
Polyclonal
unconjugated
(6 references)-
- Target See all Vaccinia Virus (VACV) products
- Vaccinia Virus (VACV) (Vaccina Virus (VACV))
-
Reactivity
- Vaccinia Virus (VACV)
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- This Vaccinia Virus antibody is un-conjugated
-
Application
- Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Purification
- Protein A Chromatography.
- Immunogen
- Lister Strain (mixture of virions and infected cell polypeptides)
-
-
- Application Notes
-
ELISA. Immunohistochemistry (Protein K digestion is recommended with Formalin-Fixed ParaffinEmbedded Sections).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 4-5 mg/mL (OD280 nm, E0.1% = 1.4)
- Buffer
- 0.01 M PBS, pH 7.2, 0.09 % Sodium Azide, None
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
-
-
: "The IgE-dependent pathway in allergic transfusion reactions: involvement of donor blood allergens other than plasma proteins." in: International journal of hematology, Vol. 102, Issue 1, pp. 93-100, (2016) (PubMed).
: "Identification and characterization of a phage display-derived peptide for orthopoxvirus detection." in: Analytical and bioanalytical chemistry, Vol. 406, Issue 29, pp. 7611-21, (2014) (PubMed).
: "Target specificity of an autoreactive pathogenic human γδ-T cell receptor in myositis." in: The Journal of biological chemistry, Vol. 287, Issue 25, pp. 20986-95, (2012) (PubMed).
: "Generation and characterization of Ptf1a antiserum and localization of Ptf1a in relation to Nkx6.1 and Pdx1 during the earliest stages of mouse pancreas development." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 56, Issue 6, pp. 587-95, (2008) (PubMed).
: "CD81 expression is important for the permissiveness of Huh7 cell clones for heterogeneous hepatitis C virus infection." in: Journal of virology, Vol. 81, Issue 10, pp. 5036-45, (2007) (PubMed).
: "Adipose tissue inflammation and increased ceramide content characterize subjects with high liver fat content independent of obesity." in: Diabetes, Vol. 56, Issue 8, pp. 1960-8, (2007) (PubMed).
-
: "The IgE-dependent pathway in allergic transfusion reactions: involvement of donor blood allergens other than plasma proteins." in: International journal of hematology, Vol. 102, Issue 1, pp. 93-100, (2016) (PubMed).
-
- Target
- Vaccinia Virus (VACV) (Vaccina Virus (VACV))
- Alternative Name
- Vaccinia Virus (VACV Products)
- Target Type
- Virus
- Background
- Vaccinia virus is an Orthopoxvirus, containing double stranded DNA. Fusion protein plays an important role in the entry of enveloped virus into cells. As vaccinia virus has a wide host range, it is conceivable that certain cellular components that are ubiquitously expressed on the cell mediate virus infection. The study of the entry process, attachment, fusion and the proteins and receptors involved is complex. During vaccinia virus infection, the fusion process is attributed to the action of the 14KDa protein (A27L). The N terminus of this protein recognises heparan sulfate on the cell surface. It interacts with the negative charges of sulfates of glycosaminoglycans (GAGs). Therefore, antibodies that recognize this 14KDa protein are able to neutralize vaccinia virus infection and enable identification other viral and cellular proteins which participate in the vaccinia virus entry process.
-
You are here:
