MAP2 antibody

Catalog No. ABIN112902

This anti-MAP2 antibody is a Rabbit Polyclonal antibody detecting MAP2 in WB and IHC (fro). Suitable for Cow.

Quick Overview for MAP2 antibody (ABIN112902)

Target: MAP2 (Microtubule-Associated Protein 2 (MAP2))

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-MAP2 Antibody

Purification: Prepared by Ammonium Sulphate fractionation

Immunogen: Native, from brain

Application Details

Application Notes: Immunohistochemistry on frozen sections (1: 5 - 1: 30). Western blot (Recognises mainly the MAP2 protein (280 kD) and to a lesser extent the Tauprotein (60 kD) in blotting using bovine brain extract. When testing against rat brainextract this antibody shows strong bands at 60 kD and fainter bands at the highermolecular weights. This could be due to differences in the sample preparation).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 25.5 mg/mL

Buffer: PBS, pH 7.2, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

Target Details for MAP2

Target: MAP2 (Microtubule-Associated Protein 2 (MAP2))

Background: MAP2 is the major microtubule associated protein of brain tissue. There are three forms of MAP2, two are similarily sized with apparent molecular weights of 280 kDa (MAP2a and MAP2b) and the third with a lower molecular weight of 70 kDa (MAP2c). In the newborn rat brain, MAP2b and MAP2c are present, while MAP2a is absent. Between postnatal days 10 and 20, MAP2a appears. At the same time, the level of MAP2c drops by 10-fold. This change happens during the period when dendrite growth is completed and when neurons have reached their mature morphology. MAP2 is degraded by a Cathepsin D-like protease in the brain of aged rats. There is some indication that MAP2 is expressed at higher levels in some types of neurons than in other types. MAP2 is known to promote microtubule assembly and to form side-arms on microtubules. It also interacts with neurofilaments, actin, and other elements of the cytoskeleton.