Shows only the La/SSB precipitate band when tested using double immunodiffusion against rabbit thymus extract. Specificity is verified by identity of precipitation with CDC-AF reference serum. The titer obtained will vary with the test system, however, undiluted antibody shows a strong La/SSB precipitate band. Product shows no reactivity at a dilution of 1/100 when tested against purified Scl-70, Sm, Sm/RNP, and Jo-1 antigens in an ELISA system designed to detect all IgG classes. Anti-SSA activity is detected in this ELISA system.
Purification
Precipitation and collection of IgG fraction from delipidized and defibrinated single donor Human plasma.
Suitable for ELISA and Immunodiffusion. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Reconstitution
Restore with 2 mL deionized water.
Buffer
0.01 M Phosphate, 0.11 M NaCl, pH 7.2 with 0.02 % Sodium Azide as preservative.
Preservative
Sodium azide
Precaution of Use
Do Not Use Sodium Azide as Preservative.
Handling Advice
Avoid repeated freezing and thawing. Dilute only prior to immediate use
Storage
4 °C/-20 °C
Storage Comment
Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
Target
SSB
(Sjogren Syndrome Antigen B (SSB))
Alternative Name
SS-B / La
Background
Synonyms: LARP3, La autoantigen, La ribonucleoprotein, Lupus La protein, SSB, Sjoegren syndrome type B antigen