Cathepsin D antibody

Catalog No. ABIN113089

This Rabbit Polyclonal antibody specifically detects Cathepsin D in WB and EIA. It exhibits reactivity toward Human.

Quick Overview for Cathepsin D antibody (ABIN113089)

Target: Cathepsin D (CTSD)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cathepsin D antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-Cathepsin D Antibody

Specificity: When tested by Immunoblot analysis at a dilution of 1/4000 the antibody reacts with Human liver Cathepsin D and does not react with Cathepsins B, H and L.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Ammonium Sulfate Precipitation

Immunogen: Mature form Cathepsin D

Application Details

Application Notes: Suitable for use in indirect ELISA (1/1000) and Immunoblotting (1/4,000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 20.625 mg/mL

Buffer: PBS, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Cathepsin D

Target: Cathepsin D (CTSD)

Alternative Name: Cathepsin D

Background: Cathepsin D is a normal lysosomal protease that is expressed in all cells. It is an aspartyl protease with a pH optimum in the range of 3-5, and contains two N-linked oligosaccharides. Cathepsin D is synthesized as an inactive 52 kDa pro enzyme. Activation involves the proteolytic removal of the 43 amino acid profragment and an internal cleavage to generate the two-chain form made up of 34 and 14 kDa subunits. Cathepsin D contains the mannose-6-phosphate lysosomal localization signal that targets the enzyme to the lysosomal compartment where it functions in the normal degradation of proteins. In certain tumor cells, Cathepsin D is abnormally processed and is secreted in its 52 kDa precursor form. Numerous clinical studies as well as in vitro evidence suggest that cathepsin D plays an important role in malignant transformation and may be a useful prognostic indicator for breast cancer and possibly Alzheimer's disease.Synonyms: CPSD, CTSD

Gene ID: 1509

NCBI Accession: NP_001900

UniProt: P07339

Pathways: Peptide Hormone Metabolism