ITGA4 antibody

Catalog No. ABIN114228

This anti-ITGA4 antibody is a Rat Monoclonal antibody detecting ITGA4 in FACS, IP, IHC (fro) and Func. Suitable for Mouse.

Quick Overview for ITGA4 antibody (ABIN114228)

Target: ITGA4 (Integrin alpha 4 (ITGA4))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This ITGA4 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Clone: R1-2

Product Details anti-ITGA4 Antibody

Specificity: This antibody reacts with alpha 4 integrin.

Purification: Protein G affinity purified immunoglobulin fraction

Immunogen: Peyers Patch HEV binding lymphoma line (TK1)

Isotype: IgG2b

Application Details

Application Notes: Flow cytometry (see protocol). Immunoprecipitation. Immunohistochemistry on frozen sections. Functional assays.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1x10e6 cells, representing 1 test). 4. To each tube, add 0. 5-1. 0 µg* of ABIN114228. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Add 100 µl of secondary antibody (FITC Goat anti-rat IgG (H+L)) at a 1/500 dilution. 9. Incubate the tubes at 4°C for 30-60 minutes. (It is recommended that the tubes are protected from light since most fluorochromes arelight sensitive). 10. Wash 2 times at 4°C in media B. 11. Resuspend the cell pellet in 50 µl ice cold media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls).

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS buffer with 0.02 % sodium azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ITGA4

Target: ITGA4 (Integrin alpha 4 (ITGA4))

Alternative Name: CD49d / ITGA4

Background: Alpha 4 integrin, which helps to mediate cell-cell and cell-matrix interactions. It combines with beta 1 and beta 7integrin to form VLA-4 and LPAM-1 (Peyers patch homing receptor) respectively. VLA-4 is expressed on most peripheral lymphocytes, thymocytes and monocytes. LPAM-1 is found on peripheral lymphocytes, but few thymocytes. Fibronectin and VCAM-1 act as ligands for both VLA-4 and LPAM-1. LPAM-1 also binds the mucosal vascular addressin MAdCAM-1. (1)Synonyms: CD49 antigen-like family member D, Integrin alpha-4, Integrin alpha-IV, VLA-4, VLA4

Gene ID: 16401

NCBI Accession: NP_034706

UniProt: Q00651

Pathways: Integrin Complex