C3 antibody

Catalog No. ABIN114403

Product Details anti-C3 Antibody

Target: C3 (Complement Component 3 (C3))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This C3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunofluorescence (IF)

Cross-Reactivity (Details): Species reactivity (tested):Mouse.

Purification: Protein G Chromatography.

Immunogen: C57BL/6 thymocytes incubated with a rat IgG2b anti-murine Thy-1 (RmT1) and C57BL/6 serum (as a source for C1q). Donor: Lou/c rat spleen cells. Fusion Partner: Mouse myeloma cell line P3X63 Ag. 8.653.

Clone: RMC11H9

Isotype: IgG2a

Application Details

Application Notes: ELISA. Western blot. Flow Cytometry. Immunofluorescence. Immunohistochemistry on Acetone Fixed Frozen Sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: FLOW CYTOMETRY ANALYSIS: 1. Prepare cell suspension in Media A. For cell preparations, deplete the red blood cellpopulation with cell separation medium . 2. Wash 2 times. 3. Resuspend cells to 1x10e6 cells in approximately 50 µl Media A in a microcentrifugetube, i. e. 50 µl of cells resuspended to 2x10e7 cells/ml (the contents of 1 tube = 1 test). 4. To each tube add 0. 5 µg of ABIN114403 per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Add 100 µl of secondary antibody (FITC Goat anti-rat IgG (H+L)) at appropriate dilution. 9. Incubate tubes at 4°C for 30-60 minutes. (It is recommended that the tubes areprotected from light since most fluorochromes are light sensitive). 10. Wash 2 times at 4°C in Media B. 11. Resuspend the cell pellet in 50 µl ice cold Media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. (This stains dead cells by intercalating DNA). Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls).

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.02 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for C3

Target: C3 (Complement Component 3 (C3))

Alternative Name: Complement C3 (C3 Products)

Synonyms: AHUS5 antibody, ARMD9 antibody, ASP antibody, C3a antibody, C3b antibody, CPAMD1 antibody, AI255234 antibody, HSE-MSF antibody, Plp antibody, C3 antibody, C3-1 antibody, sb:cb26 antibody, si:dkey-76b14.4 antibody, wu:fi34b03 antibody, wu:fj86e11 antibody, C3d antibody, asp antibody, cpamd1 antibody, c3 antibody, complement C3 antibody, complement component 3 antibody, complement component c3a, duplicate 1 antibody, complement component 3 L homeolog antibody, complement component C3 antibody, complement C3-like antibody, C3 antibody, c3a.1 antibody, c3.L antibody, LOC443475 antibody, c3 antibody, c3-1 antibody, LOC100060539 antibody

Background: C3 is the most abundant complement protein in serum. C3 and its cleavage products, C3a and C3b, play a central role in the complement activation cascade. C3b forms an integral part of the C3 and C5 convertases as it promotes complement activation and the subsequent formation of the membrane attack complex. C3a possesses anaphlatoxic as well as various immunoregulatory properties. Also, C3 has been implicated in developmental and non-imflammatory process such as hematopoiesis, skeletal and vascular development and reproduction.Synonyms: C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, CPAMD1, Complement component 3

Gene ID: 10090

UniProt: P01027

Pathways: Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling