NEFL antibody

Catalog No. ABIN114492

The Mouse Monoclonal anti-NEFL antibody (Clone 2F11) (ABIN114492) specifically detects NEFL in WB, IHC (p) and IHC (fro). The antibody is reactive with Human, Rat, Mouse, Opossum, Cat and Rabbit samples.

Quick Overview for NEFL antibody (ABIN114492)

Target: NEFL (Neurofilament, Light Polypeptide (NEFL))

Reactivity: Human, Rat, Mouse, Opossum, Cat, Rabbit

Host: Mouse

Clonality: Monoclonal

Conjugate: This NEFL antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 2F11

Product Details anti-NEFL Antibody

Specificity: 2F11 reacts exclusively with the phosphorylated isoform of the 70 kDa neurofilament protein. This antibody labels neurons, neuronal processes, and peripheral nerves as well as sympathetic ganglion cells and adrenal medulla. Cell body of neurons, containing the non-phosphorylated neurofilament, is weakly stained.

Cross-Reactivity (Details): Also reacts to: Neurofilament H

Purification: Purified

Immunogen: Human neurofilament preparation

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry on frozen and paraffin embedded sections: This antibody may bediluted to a titer of 1: 50-1: 200 in an ABC method. Immunoblotting: 1: 200 -1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2-8 °C for one month or (in small aliquots) at -20 °C for longer.

Target Details for NEFL

Target: NEFL (Neurofilament, Light Polypeptide (NEFL))

Alternative Name: Neurofilament L (68kDa)

Background: Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. The neurofilament NF) triplet proteins (70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are normally restricted to neurons. The 70 kDa NF-protein can self-assemble into a filamentous structure, whereas the 160 kDa and 200 kDa NF-proteins require the presence of the 70 kDa NF-protein to co-assemble. All three NF proteins can be detected by immunohistochemical methods at day 9 or 10 after gestation in the mouse embryo. Although IFPs of the neurofilament type are normally restricted to neurons, there are reports on their expression in non-neuronal cells as well. For example, in heart conduction myocytes NF proteins are expressed together with desmin. In tumorpathology ganglioneuroblastomas and some of the other neuroblastomas are strongly positive with the neurofilament antisera. Also, some neuro-endocrine malignancies may show NF positivity. In cell cultures of neural tissues the neurofilament antibodies can monitor in vitro differentiationSynonyms: 68 kDa neurofilament protein, NEFL, NF-L, NF68, Neurofilament L

Molecular Weight: 68 kDa and 208 kDa (phosphorylated forms of these subunits).

Gene ID: 4747

NCBI Accession: NP_006149

UniProt: P07196