EGF antibody

Catalog No. ABIN114522

The Mouse Monoclonal anti-EGF antibody (Clone S-134) (ABIN114522) specifically detects EGF in ELISA (Capture). The antibody is reactive with Human samples.

Quick Overview for EGF antibody (ABIN114522)

Target: EGF (Epidermal Growth Factor (EGF))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EGF antibody is un-conjugated

Application: ELISA (Capture)

Clone: S-134

Product Details anti-EGF Antibody

Specificity: This antibody reacts with natural and recombinant human EGF. Does not cross react with other Human cytokines tested such as IL-1 beta, IL-8, MCAF, TGF-beta and SAA.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatograpghy on Protein G.

Immunogen: Purified recombinant Human EGF, 6kDa, 53 amino acid residues

Isotype: IgG1

Application Details

Application Notes: ELISA: This antibody can be used as a Capture antibody in sandwich ELISA applications forHuman EGF detection in combination with a monoclonal tracer/detection antibody. Suggested Capture Coating Dose: 0.3 μg/well, Substrate: TMB. If the above suggestedconditions are followed approximately 20 pg/mL of EGF in serum/plasma or 15 pg/mL ofEGF in cell medium/urine can be detected with an assay range of 0-1000 pg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with double distillated water is recommended to adjust the final concentration to 1.00 mg/mL

Concentration: 1.00 mg/mL

Buffer: 0.01 M PBS, pH 7.2 without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody at -20 °C. Can be shipped at 2 - 8 °C.

Target Details for EGF

Target: EGF (Epidermal Growth Factor (EGF))

Alternative Name: EGF

Background: Epidermal growth factor (EGF) has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. EGF exerts its actions by binding to the EGFR, a 170 kDa protein. Epidermal growth factor (EGF) is a key growth factor regulating cell survival. Through its binding to cell surface receptors, EGF activates an extensive network of signal transduction pathways that include activation of the PI3K/AKT, RAS/ERK and JAK/STAT pathways. Because of its key role in driving the proliferation of cells, EGFR is a target of several anti-cancer drugs currently in development.Synonyms: Epidermal growth factor, HOMG4, Pro-epidermal growth factor, URG, Urogastrone

Gene ID: 1950

NCBI Accession: NP_001954

UniProt: P01133

Pathways: NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation