GM-CSF antibody

Catalog No. ABIN114524

The Mouse Monoclonal anti-GM-CSF antibody is suitable to detect GM-CSF in samples from Human. It has been validated for WB and ELISA (Capture).

Quick Overview for GM-CSF antibody (ABIN114524)

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GM-CSF antibody is un-conjugated

Application: Western Blotting (WB), ELISA (Capture)

Clone: 59

Product Details anti-GM-CSF Antibody

Specificity: This monoclonal antibody reacts with natural and recombinant Human GM-CSF. Does not show any cross reaction with other Human Cytokines or Growth Factors tested such as M-CSF, G-CSFR, IL-8, IL-16, IL1-beta, TGF beta-1 and TNF-alpha.

Purification: Affinity Chromatography on Protein G.

Immunogen: Purified recombinant human GM-CSF Mouse IgG1.

Isotype: IgG1

Application Details

Application Notes: ELISA: This antibody can be used as a capture antibody in sandwich ELISA applications forhuman GM-CSF detection in combination with a monoclonal tracer/detection antibody. Suggested Capture Coating Dose: 0.3 μg/well, Substrate: TMB. If the above suggestedconditions are followed approximately 2 pg/mL of GM-CSF in serum/plasma or medium canbe detected with an assay range of 0-500 pg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with double distillated water to adjust the final concentration to 1.00 mg/mL

Concentration: 1.0 mg/mL

Buffer: 0.01 M PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for GM-CSF

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Alternative Name: GM-CSF

Background: The cytokine GM-CSF stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. Used in myeloid reconstitution following bone marrow transplant, bone marrow transplant engraftment failure or delay, mobilization and following transplantation of autologous peripheral blood progenitor cells, and following induction chemotherapy in older adults with acute myelogenous leukemia.Synonyms: CSF2, Colony stimulating factor 2, GMCSF, Granulocyte-macrophage colony-stimulating factor, Molgramostin, Sargramostim

Gene ID: 1437

NCBI Accession: NP_000749

UniProt: P04141

Pathways: JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin