IL-8 antibody

Catalog No. ABIN114532

This Mouse Monoclonal antibody specifically detects IL-8 in WB, Func, EIA and ELISA (Capture). It exhibits reactivity toward Human.

Quick Overview for IL-8 antibody (ABIN114532)

Target: IL-8 (IL8) (Interleukin 8 (IL8))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL-8 antibody is un-conjugated

Application: Western Blotting (WB), Functional Studies (Func), Enzyme Immunoassay (EIA), ELISA (Capture)

Clone: I8-S2

Product Details anti-IL-8 Antibody

Purification: Affinity Chromatography on Protein G.

Immunogen: Purified recombinant Human IL-8.

Isotype: IgG2b

Application Details

Application Notes: ELISA: Reacts with human IL-8 (matched pair with clone I8-60, DM1023). Neutralizing: In chemotaxis assay, the antibody inhibited 97.8 % % of the chemotacticactivity of IL-8 on RB/293 cell using 5 μg/mL of MAb and 10 ng/mL of human IL-8 in assay. This MAb inhibited the chemotactic effect of human IL-8, had no inhibitory effect to MIP1-beta and RANTES in neutrophil chemotaxis assay. It indicates the MAb is specialneutralizing the IL-8 activity. The MAb has been shown the neutralizing activity on calcium ion changes in humangranulocytes using FACS. Western Blot: Concentration of 0.02-0.1 μg/mL MAb will allow visualization of 100 ng/laneof human IL-8.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Double distillated water is recommended to adjust the final concentration to 1.0 mg/mL.

Buffer: 0.01 M PBS, pH 7.0

Preservative: Azide free

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2-8 °C for one month or at -20 °C for longer.

Target Details for IL-8

Target: IL-8 (IL8) (Interleukin 8 (IL8))

Alternative Name: Interleukin-8 / IL8

Background: Interleukin 8 (IL-8), formerly called monocyte-derived neutrophil chemotactic factor, belongs to the C-X-C chemokine family. IL-8 has 4 cysteine residues, as do other members of the chemokine family, and the first two cysteine residues are separated by glutamine. IL-8 consists of 72 amino acids with a molecular weight of 8,000 daltons. IL-8 exhibits chemotactic activity in vitro for T cells, basophils and neutrophils. IL-8 activates neutrophils to release lysosomal enzymes including myeloperoxidase, -mannosidase and -glucuronidase. IL-1 induces the production of IL-8 from fibroblasts, keratinocytes, endothelial cells, hepatoma cells, astrocytoma cells, glioblastoma cells, lung epithelial cells, synovial membrane cells, melanocytes, melanoma cells and gastric cancer cells. Lipopolysaccharides will stimulate IL-8 production in monocytes/macrophages, and expressed on endothelial cells in response to inflammation.Synonyms: CXCL8, Emoctakin, GCP1, Granulocyte chemotactic protein 1, MDNCF, MONAP, Monocyte-derived neutrophil chemotactic factor, Monocyte-derived neutrophil-activating peptide, NAP1, Neutrophil-activating protein 1, Protein 3-10C, T-cell chemotactic factor

Gene ID: 9606

UniProt: P10145

Pathways: TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Hepatitis C, Autophagy