CDC25A antibody

Catalog No. ABIN115127

The Mouse Monoclonal anti-CDC25A antibody is suitable to detect CDC25A in samples from Human and Rat. It has been validated for WB, IF, IHC (p) and IP.

Quick Overview for CDC25A antibody (ABIN115127)

Target: CDC25A (Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CDC25A antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Clone: DCS-120-DCS-121

Product Details anti-CDC25A Antibody

Specificity: This antibody reacts with a 65 kDa protein known as CDC25A.
Cellular Localization: Nuclear membrane.

Purification: Purified

Immunogen: BALB/C mice were injected with purified recombinant CDC25A.

Isotype: IgG2a

Application Details

Application Notes: Immunflourescence. Immunoprecipitation. Western Blot. Immunohistochemistry on Paraffin Embedded Sections: Use a dilution of 1/50-1/100 in an ABC method (30 minutes at room temperature, requireshigh temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior toimmunostaining)Recommended Positive Control: Tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CDC25A

Target: CDC25A (Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))

Alternative Name: CDC25A

Background: The activity of cyclin-dependent kinases in cell cycle is regulated by the phosphorylation status, which is controlled by the antagonistic action of wee 1 kinase and CDC25 phosphatases. Three CDC25 genes are present in human cells: CDC25A, CDC25B and CDC25C. CDC25A and CDC25B are expressed throughout the cell cycle, with peak expression in G1 for CDC25A and in both G1-S phase and G2 for CDC25B. CDC25C is mainly expressed in G2 phase.Synonyms: CDC-25A, Cell Division Cycle 25A, Dual specificity phosphatase Cdc25A, M-phase inducer phosphatase 1

Gene ID: 993

NCBI Accession: NP_001780

UniProt: P30304

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, M Phase