MCM7 antibody

Catalog No. ABIN115133

The Mouse Monoclonal anti-MCM7 antibody has been validated for WB, IHC (p), IP and IHC (fro). It is suitable to detect MCM7 in samples from Human.

Quick Overview for MCM7 antibody (ABIN115133)

Target: MCM7 (Minichromosome Maintenance Complex Component 7 (MCM7))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MCM7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 47DC141

Product Details anti-MCM7 Antibody

Specificity: This antibody reacts with a 80 kDa protein known as hCDC47.
Cellular Localization: Nuclear

Purification: Purified

Immunogen: BALB/C mice were injected with recombinant human CDC47 protein.

Isotype: IgG1

Application Details

Application Notes: Immunoprecipitation. Western Blot. Immunohistochemistry on paraffin embedded sections: diluted of 1: 50-1: 100 in an ABC method (30 minutes at room temperature)Recommended Positive Control: Breast carcinoma
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM citrate buffer, pH 6.0 - containing sodium azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for MCM7

Target: MCM7 (Minichromosome Maintenance Complex Component 7 (MCM7))

Alternative Name: MCM7

Background: HCDC47 is a member of MCM family, which allows DNA to replicate once per cell cycle. Expression of hCDC47 protein has been found in the nuclei of proliferative components of normal lymph nodes, bone marrow, epidermis and mucosa. Malignant tumors from several organs contain more nuclear hCDC47-positive cells than their normal counterparts.Synonyms: CDC47, CDC47 homolog, DNA replication licensing factor MCM7, MCM2, P1.1-MCM3, minichromosome maintenance complex component 7

Gene ID: 4176

NCBI Accession: NP_005907

UniProt: P33993

Pathways: DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA