Recombinant MCM7 antibody (AA 195-319)

Catalog No. ABIN7477515

Product Details anti-MCM7 Antibody

Target: MCM7 (Minichromosome Maintenance Complex Component 7 (MCM7))

Antibody Type: Recombinant Antibody

Binding Specificity: AA 195-319

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This MCM7 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Immunogen: Recombinant human MCM7 protein fragment (aa195-319)

Clone: MSVA-507R

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Colon: A strong nuclear MCM7 immunostaining should be seen in virtually all crypt base cells.

Negative Control: Colon: MCM7 immunostaining should be largely absent in surface epithelial cells and in most stroma cells.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for MCM7

Target: MCM7 (Minichromosome Maintenance Complex Component 7 (MCM7))

Alternative Name: MCM7 (MCM7 Products)

Synonyms: CDC47 antibody, MCM2 antibody, P1.1-MCM3 antibody, P1CDC47 antibody, P85MCM antibody, PNAS146 antibody, MCM7 antibody, AI747533 antibody, Mcmd7 antibody, mCDC47 antibody, chunp6911 antibody, nyz175 antibody, sr:nyz175 antibody, cdc47 antibody, cdc47-2 antibody, mcm7 antibody, xmcm7 antibody, CG4978 antibody, DmMCM7 antibody, DmeMCM7 antibody, Dmel\\CG4978 antibody, McM7 antibody, Mcp-PCR1 antibody, PCR1 antibody, anon-EST:Liang-1.66 antibody, clone 1.66 antibody, minichromosome maintenance complex component 7 antibody, mini-chromosome maintenance complex protein 7 antibody, minichromosome maintenance complex component 2 antibody, minichromosome maintenance complex component 7 L homeolog antibody, Minichromosome maintenance 7 antibody, minichromosome maintenance complex component 7 S homeolog antibody, MCM complex subunit Mcm7 antibody, MCM7 antibody, CDC47 antibody, Mcm7 antibody, MCM2 antibody, mcm7 antibody, mcm7.L antibody, mcm7.S antibody

Background: CDC47, DNA replication licensing factor MCM7, MCM7 mini chromosome maintenance deficient 7, Minichromosome Maintenance 7, Mini chromosome maintenance protein 7, P1.1-MCM3, P1CDC47, P85MCM, PNAS146,MCM7 antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P33993

Pathways: DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA