MSH6 antibody

Catalog No. ABIN115271

The Mouse Monoclonal anti-MSH6 antibody is suitable to detect MSH6 in samples from Human, Mouse, Rat and Dog. It has been validated for WB, IHC (p), IP and IHC (fro).

Quick Overview for MSH6 antibody (ABIN115271)

Target: MSH6 (MutS Homolog 6 (E. Coli) (MSH6))

Reactivity: Human, Mouse, Rat, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This MSH6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 44

Product Details anti-MSH6 Antibody

Specificity: MSH6 is a heterodimer of MSH2 binds to DNA containing G/T mismatches. The MSH2-MSH6 complex recognizes a single-based mispair insertion/deletion loop.
Cellular Localization: Nuclear.

Purification: Affinity Chromatography.

Immunogen: Synthetic human MSH6 peptide

Isotype: IgG1

Application Details

Application Notes: Immunoprecipitation. Western Blot: 1/100-1/500. Immunohistochemistry on Formalin-Fixed Paraffin Embedded Sections: 1/25-1/50 in an ABCmethod (30 to 60 minutes at room temperature (requires high temperature antigenunmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining). Immunohistochemistry on Frozen Sections. Recommended Positive Control: Tonsil, colon carcinoma
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for MSH6

Target: MSH6 (MutS Homolog 6 (E. Coli) (MSH6))

Alternative Name: MSH6

Background: An alteration of microsatellite repeats is the result of slippage owing to strand misalignment during DNA replication and is referred to as microsatellite instability (MSI). These defects in DNA repair pathways have been related human carcinogenesis. Studies have shown the mutations of MLH-1, MSH2 and MSH6 genes contribute to the development of sporadic colorectal carcinoma. The repair of mismatch DNA is essential to maintaining the integrity of genetic information over time.Synonyms: DNA mismatch repair protein Msh6, G/T mismatch-binding protein, GTBP, MutS-alpha 160 kDa subunit, p160

Gene ID: 2956

NCBI Accession: NP_000170

UniProt: P52701

Pathways: DNA Damage Repair, Chromatin Binding, Production of Molecular Mediator of Immune Response