Cardiotrophin 1 antibody

Catalog No. ABIN115501

This Rabbit Polyclonal antibody specifically detects Cardiotrophin 1 in WB and EIA. It exhibits reactivity toward Human.

Quick Overview for Cardiotrophin 1 antibody (ABIN115501)

Target: Cardiotrophin 1 (CTF1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cardiotrophin 1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-Cardiotrophin 1 Antibody

Sequence: MRGSHHHHHH GSSRREGSLE DPQTDSSVSL LPHLEAKIRQ THSLAHLLTK YAEQLLQEYV QLQGDPFGLP SFSPPRLPVA GLSAPAPSHA GLPVHERLRL DAAALAALPP LLDAVCRRQA ELNPRAPRLL RRLEDAARQA RALGAAVEAL LAALGAANRG PRAEPPAATA SAASATGVFP AKVLGLRVCG LYREWLSRTE GDLGQLLPGG SA

Specificity: This antibody recognizes Human Cardiotrophin-1. Other species not tested.

Purification: Immunoaffinity chromatography

Immunogen: Recombinant Human Cardiotrophin-1.

Application Details

Application Notes: ELISA. Western Blot.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.1 mL of deionized water. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.

Concentration: 1.0 mg/mL

Buffer: 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2. AZIDE FREE, None

Preservative: Azide free

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Prior to reconstitution store at -70 °C. Following reconstitution store the antibody (in aliquots) at -20 °C for 6 months without detectable loss of activity.

Expiry Date: 6 months

Target Details for Cardiotrophin 1

Target: Cardiotrophin 1 (CTF1)

Alternative Name: Cardiotrophin-1

Background: Cardiotrophin-1 (CT-1) is a 201 amino acid member of the interleukin-6 superfamily. It was identified by its ability to induce hypertrophic response in cardiac myocytes. CT-1 mRNA levels were found both in cardiac myocytes and in cardiac nonmyocytes. CT-1 was also detected in abundance in normal adult human lung and was expressed in both fetal and adult airway smooth muscle cells. CT-1activates gp130 dependent signaling and stimulates the Janus kinase/signal transducers and activators of transcription (JAK/STAT) pathway to transduce hypertrophic and cytoprotective signals in cardiac myocytes. CT-1 has also a neurotrophic function. CT-1 deficiency causes increased motoneuron cell death in spinal cord and brainstem nuclei of mice during a period between embryonic day 14 and the first postnatal week. Moreover, CT-1 is a hepatocyte survival factor that efficiently reduces hepatocellular damage in animal models of acute liver injury. CT-1 expression is augmented after hypoxic stimulation and it can protect cardiac cells when added either prior to simulated ischaemia or at the time of reoxygenation following simulated ischaemia. CT-1 can induce expression of the protective heat shock proteins (hsps) in cardiac cells. Cardiotrophin-1 increased ventricular expression of ANP, brain natriuretic peptide (BNP) and angiotensinogen mRNA. CT-1 levels were significantly elevated in patients with heart failure, patients with dilatative cardiomyopathy, moderate/severe mitral regurgitation, stable and unstable angina and after acute myocardial infarction. Total 212 AA. MW: 22.5 kDa (calculated). N-Terminal His-tag, 12 extra AA (highlighted).Synonyms: CT-1, CTF1

Gene ID: 1489

NCBI Accession: NP_001136016

UniProt: Q16619

Pathways: JAK-STAT Signaling