Pleiotrophin antibody

Catalog No. ABIN115512

Product Details anti-Pleiotrophin Antibody

Target: Pleiotrophin (PTN)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Pleiotrophin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Sequence: MKHHHHHHHM LVPRGSGKKE KPEKKVKKSD CGEWQWSVCV PTSGDCGLGT REGTRTGAEC KQTMKTQRCK IPCNWKKQFG AECKYQFQAW GECDLNTALK TRTGSLKRAL HNAECQKTVT ISKPCGKLTK PKPQAESKKK KKEGKKQEKM LD

Specificity: This antibody reacts to Pleiotrophin.

Purification: Immunoaffinity chromatography

Immunogen: Recombinant human OSF-1 (E.coli)

Application Details

Application Notes: ELISA. Western blot. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.05 mL of deionized water.

Concentration: 1.0 mg/mL

Buffer: 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2. AZIDE FREE

Preservative: Azide free

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store (in aliquots) at -20 °C.

References for anti-Pleiotrophin antibody (ABIN115512)

Asari, Kanemitsu, Kurihara: "Oral administration of high molecular weight hyaluronan (900 kDa) controls immune system via Toll-like receptor 4 in the intestinal epithelium." in: The Journal of biological chemistry, Vol. 285, Issue 32, pp. 24751-8, (2010) (PubMed).

Target Details for Pleiotrophin

Target: Pleiotrophin (PTN)

Alternative Name: Pleiotrophin / PTN (PTN Products)

Synonyms: zHBNF antibody, zgc:111787 antibody, harp antibody, hbnf antibody, X-PTF antibody, hbgf8 antibody, negf1 antibody, ptn antibody, PTN antibody, HARP antibody, HBGF8 antibody, HBNF antibody, NEGF1 antibody, PTN-A antibody, HB-GAM antibody, HBBM antibody, HBGF-8 antibody, OSF-1 antibody, HBBN antibody, OSF antibody, Osf-1 antibody, Osf1 antibody, Hbnf antibody, pleiotrophin antibody, pleiotrophin L homeolog antibody, ptn antibody, PTN antibody, ptn.L antibody, Ptn antibody

Background: Pleiotrophin (Osteoblast-Specific Factor-1, OSF-1) contains 136 amino acid residues. The sequence is very rich in cationic amino acids (24 % of the residues), lysine cluster sequences are found in the N-terminal and Cterminal ends of the structure. The OSF-1 gene was shown by Northern blotting analysis to be expressed in mouse calvarial osteoblastenriched cells and in mouse brain tissues, but not in thymus, spleen, kidney, liver, lung, testis or heart. Pliotrophin has the ability to promote adhesion, migration, expansion, and differentiation of human osteoprogenitor cells. In addition to certain types of cancer, the embryonic growth and differentiation factor pleiotrophin is found also in adults in inflammatory diseases. In osteoarthritis, pleiotrophin is especially expressed in early stages, and its concentrations in the synovial fluid could serve as a marker for the progress of the disease. Pleitrophin might be involved in cartilage repair in osteoarthritis, in particular, in earlier stages. Total 152 AA. MW: 17.3 kDa. N-Terminal His-tag and thrombin cleavage site 16 AA (highlighted).Synonyms: HB-GAM, HBBM, HBGF-8, HBNF-1, HBNF1, Heparin-binding brain mitogen, Heparin-binding growth factor 8, Heparin-binding growth-associated molecule, Heparin-binding neurite outgrowth-promoting factor 1, NEGF1, OSF-1, OSF1, Osteoblast-specific factor 1

Gene ID: 5764

NCBI Accession: NP_002816

UniProt: P21246

Pathways: RTK Signaling