IL1A antibody (Biotin)

Catalog No. ABIN115859

The Rabbit Polyclonal anti-IL1A antibody is suitable to detect IL1A in samples from Mouse. It has been validated for WB and EIA.

Quick Overview for IL1A antibody (Biotin) (ABIN115859)

Target: IL1A (Interleukin 1 alpha (IL1A))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL1A antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-IL1A Antibody (Biotin)

Specificity: This antibody reacts with Mouse Interleukin-1 alpha (IL-1 alpha).

Purification: Affinity chromatography

Immunogen: Highly pure (>98%) recombinant mIL-1 alpha

Application Details

Application Notes: ELISA: (Direct): To detect mIL-1α by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonalantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2- 0.4 ng/well of recombinant mIL-1α. (Sandwich): To detect mIL-1α by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonalantibody, in conjunction with Polyclonal Anti-Murine IL-1α (PP005P) as a capture antibody,allows the detection of at least 0.2-0.4 ng/well of recombinant mIL-1α. Western blot: To detect mIL-1α by Western blot analysis this antibody can be used at a concentration of0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant mIL-1α is 1.5-3.0 ng/lane, under either reducing or non-reducing

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for IL1A

Target: IL1A (Interleukin 1 alpha (IL1A))

Alternative Name: Interleukin-1 alpha / IL-1A

Background: Interleukins (ILs) are a large group of cytokines that are produced mainly by leukocytes, although some are produced by certain phagocytes and auxiliary cells. Each IL acts on a specific, limited group of cells through a receptor specific for that IL. Interleukin 1 (IL1), originally known as lymphocyte activating factor (LAF), activates T cells and lymphocytes, which then proliferate and secrete interleukin 2. IL1 is primarily released from stimulated macrophages and monocytes, but also is released from several other cell types and is thought to play a key role in inflammatory and immune responses. The two closely related agents, interleukin 1 alpha (IL1 alpha) and interleukin 1 beta (IL1 beta) bind to the same cell surface receptor, elicit nearly identical biological responses and share 25 % homology in their amino acid sequence.Synonyms: Hematopoietin-1, IL-1 alpha, IL1A, IL1F1

Gene ID: 16175

NCBI Accession: NP_034684

UniProt: P01582

Pathways: NF-kappaB Signaling, Autophagy, Cancer Immune Checkpoints