IL-6 antibody (Biotin)

Catalog No. ABIN115959

The Biotin-conjugated Rabbit Polyclonal anti-IL-6 antibody (ABIN115959) specifically detects IL-6 in WB and EIA. The antibody is reactive with Rat samples.

Quick Overview for IL-6 antibody (Biotin) (ABIN115959)

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-6 antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-IL-6 Antibody (Biotin)

Specificity: This antibody recognises Rat Interleukin-6 (rIL-6)

Purification: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant rat IL-6.

Application Details

Application Notes: ELISA: To detect rIL-6 by direct ELISA (using 100 l/well antibody solution) this antibody can beused at a concentration of 0.15-0.30 μg/mL. Used in conjunction with compatiblesecondary reagents, allows the detection of at least 0.2 ng/well of recombinant rIL-6. Western Blot: To detect rIL-6 by Western Blot analysis this antibody can be used at a concentration of0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant rIL-6 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for IL-6

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Alternative Name: Interleukin-6 (IL6)

Background: Interleukin 6 (IL6) is a multifunctional 24 kD protein originally discovered in the medium of RNA stimulated fibroblastoid cells. It is upregulated by IL1, TNF, PDGF, IFN beta, TNF alpha, NGF, IL17 and downregulated by glucocorticoids IL4, TGF beta. IL6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be as important as IL1 and TNF alpha in regulating the acute phase response. IL6 has also been implicated in regulating adipose mass. IL6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages and endothelial cells. It acts upon a variety of cells including fibroblasts, myeloid progenitor cells, T cells, B cells and hepatocytes. In addition, IL6 appears to interact with IL2 in the proliferation of T lymphocytes. IL6 potentiates the proliferative effect of IL3 on multipotential hematopoietic progenitors.Synonyms: B-cell stimulatory factor 2, BSF-2, CDF, CTL differentiation factor, Hybridoma growth factor, IFNB2, IL-6, Interferon beta-2

Gene ID: 24498

NCBI Accession: NP_036721

UniProt: P20607

Pathways: TLR Signaling, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome