FLT3LG antibody

Catalog No. ABIN116014

This Rabbit Polyclonal antibody specifically detects FLT3LG in WB, EIA and Func. It exhibits reactivity toward Human.

Quick Overview for FLT3LG antibody (ABIN116014)

Target: FLT3LG (Fms-Related tyrosine Kinase 3 Ligand (FLT3LG))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FLT3LG antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Product Details anti-FLT3LG Antibody

Specificity: Human flt3-Ligand.

Purification: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant hflt3-Ligand

Application Details

Application Notes: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhFlt-3 Ligand (15 ng/mL), a concentration of 0.25-0.75 μg/mL of this antibody is required. ELISA: (Indirect): To detect hFlt-3 Ligand by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant hFlt-3 Ligand. (Sandwich): To detect hFlt-3 Ligand by sandwich ELISA (using 100 μL/well antibodysolution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with Biotinylated Anti-Human Flt-3 Ligand (PP1008B1,PP1008B2) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well ofrecombinant hFlt-3 Ligand. Western Blot: To detect hFlt-3 Ligand by Western Blot analysis this antibody can be used ata concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hFlt-3 Ligand is 1.5-3.0 ng/lane, under either reducingor non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Centrifuge vial prior to opening! This product is photosensitive and should be protected from light

Storage: 4 °C

Storage Comment: Store the antibody at 2-8 °C. DO NOT FREEZE!

Target Details for FLT3LG

Target: FLT3LG (Fms-Related tyrosine Kinase 3 Ligand (FLT3LG))

Alternative Name: Flt3 Ligand

Background: Flt3 ligand is a ligand for the FLT3 tyrosine kinase receptor and belongs to a small group of growth factors that regulate proliferation of early hematopoietic cells. Multiple isoforms of Flt3 ligand have been identified. The predominant form is the transmembrane form, which is biologically active on the cell surface. When proteolytically cleaved the transmembrane isoform generates a soluble form, which is also biologically active. Flt3 ligand binds to cells expressing the tyrosine kinase receptor Flt3. Flt3 ligand alone cannot stimulate proliferation, but synergizes well with other CSFs and interleukins to induce growth and differentiation.Synonyms: FLT3LG, Flt3L, SL cytokine, fms-related tyrosine kinase 3 ligand

Gene ID: 2323

NCBI Accession: NP_001191431

UniProt: P49771

Pathways: RTK Signaling