GDNF antibody (Biotin)

Catalog No. ABIN116019

This anti-GDNF antibody is a Rabbit Polyclonal antibody detecting GDNF in WB, IHC (fro) and EIA. Suitable for Human.

Quick Overview for GDNF antibody (Biotin) (ABIN116019)

Target: GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GDNF antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Product Details anti-GDNF Antibody (Biotin)

Specificity: Human Glial Derived Neurotrophic Factor.

Purification: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant hGDNF.

Application Details

Application Notes: ELISA: To detect hGDNF by direct ELISA (using 100 ml/well antibody solution) this GDNFantibody can be used at a concentration of 0.15-0.30 μg/mL. Used in conjunction withcompatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinanthGDNF. Western Blot: To detect hGDNF by Western Blot analysis this GDNF antibody can be used ata concentration of 0.1-0.2 mg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hGDNF is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Centrifuge vial prior to opening! This product is photosensitive and should be protected from light

Storage: 4 °C

Storage Comment: Store the antibody at 2-8 °C. DO NOT FREEZE!

Target Details for GDNF

Target: GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))

Alternative Name: GDNF

Background: Glial cell line derived neurotrophic factor (GDNF) is a highly conserved neurotrophic factor that is a distant member of the TGF beta superfamily. The GDNF gene product is processed to a disulphide-linked homodimer, which is a ligand for the RET protooncogene. GDNF protects and repairs dopamine-containing neurons, which degenerate in Parkinson's disease, and motor neurons, which die in amyotrophic lateral sclerosis. The use of GDNF in the treatment of Parkinson's disease has shown promise in the clinic. Treatment of spinal cord injuries with GDNF has also produced neurological improvement.Synonyms: ATF, Astrocyte-derived trophic factor, Glial cell line-derived neurotrophic factor

Gene ID: 2668

NCBI Accession: NP_000505

UniProt: P39905

Pathways: RTK Signaling, Synaptic Membrane, Tube Formation, Autophagy, Smooth Muscle Cell Migration