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GDNF antibody (Glial Cell Line Derived Neurotrophic Factor) AA 121-211 Primary Antibody

GDNF Reactivity: Human, Mouse, Rat, Cow, Rabbit ELISA, FACS, IF (cc), IF (p), IHC (fro), IHC (p), WB Host: Rabbit Polyclonal unconjugated
Pubmed (1 reference) Independent Validation (1 validation)
Catalog No. ABIN736536
$374.00
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100 μL
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  • Target See all GDNF Antibodies
    GDNF
    Binding Specificity
    • 15
    • 10
    • 9
    • 7
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 121-211
    Reactivity
    • 89
    • 48
    • 46
    • 15
    • 15
    • 1
    Human, Mouse, Rat, Cow, Rabbit
    Host
    • 85
    • 7
    • 3
    • 3
    Rabbit
    Clonality
    • 86
    • 13
    Polyclonal
    Conjugate
    • 47
    • 11
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This GDNF antibody is un-conjugated
    Application
    • 76
    • 35
    • 31
    • 21
    • 19
    • 15
    • 12
    • 12
    • 8
    • 7
    • 4
    • 4
    • 4
    • 3
    • 1
    • 1
    • 1
    ELISA, Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
    Predicted Reactivity
    Dog,Cow,Pig,Horse,Chicken
    Purification
    Purified by Protein A.
    Immunogen
    KLH conjugated synthetic peptide derived from human GDNF
    Isotype
    IgG
  • Application Notes
    WB 1:300-5000
    ELISA 1:500-1000
    FCM 1:20-100
    IHC-P 1:200-400
    IHC-F 1:100-500
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200
    Restrictions
    For Research Use only
  • Validation #102003 (Immunofluorescence)
    'Independent Validation' Badge
    by
    Prof. Merighi, Laboratory of Neurobiology, Department of Veterinary Sciences, University of Turin
    No.
    #102003
    Date
    02/12/2018
    Antigen
    GDNF
    Lot Number
    9F01M8
    Method validated
    Immunofluorescence
    Positive Control
    mouse hippocampus and putamen
    Negative Control
    no primary control
    Notes

    ABIN736536 works in IF albeit with some background staining that we have been unable to eliminate although using different dilutions to increase the signal-to-noise ratio.

    'Independent Validation' Badge
    Validation Images
    Full Methods
    Primary Antibody
    ABIN736536
    Secondary Antibody
    anti-rabbit AF488 conjugated antibody (Life Technologies)
    Full Protocol
    • Perfuse mouse with 4% paraformaldehyde in 0.1M phosphate buffer (PB) pH7.4.
    • Post-fix spinal cord and brain blocks in the same fixative for additional 2h at RT.
    • Wash spinal cord and brain blocks several times with PBS.
    • Cut blocks with a vibratome (Leica, VT1000 S) into 70µm thick transverse sections.
    • Cut dorsal root ganglions (DRGs) with a cryostat into 17µm thick sections after cryoprotection and glass mounting.
    • Block free floating vibratome and glass mounted cryostat sections with blocking solution (0.01M PBS5% Normal Goat Serum (NGS; Sigma, G9023, lot SLBV1396), 0.1% Triton X-100 (BioRad, 161-0407, lot 00583) for 1h at RT.
    • Incubate sections with primary rabbit anti-GDNF antibody (antibodies-online, ABIN736536, lot 9F01M8) diluted 1:100 in blocking solution ON at RT.
    • Wash sections 4x for 5min with 0.01M PBS.
    • Incubate sections with secondary goat anti-rabbit AF488 conjugated antibody (Life Technologies) diluted 1:500 in PBS for 1h at RT.
    • Wash sections 4x for 5min with 0.01M PBS.
    • Mount sections in Fluoroshield (Sigma, F6182, lot MKCB0153V).
    Experimental Notes
    • Different dilutions were also tested (1:200, 1:500, 1:2000) with or without Triton-X but the 1:100 dilution and the use of Triton-X in the blocking solution gave the best results.

    • Staining is mainly present in the cytoplasm of small-size neurons in the DRG, consistent with what has been previously described. ABIN736536 stains fibers in superficial laminae of the dorsal horn of the spinal cord. In the hippocampus staining is present in the CA1 both in the pyramidal neurons (cell body and dendrites) and in individual interneurons. In the putamen ABIN736536 stains neuron cell bodies and some dendrites.

  • Format
    Liquid
    Concentration
    1 μg/μL
    Buffer
    0.01M TBS( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
    Precaution of Use
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
    Expiry Date
    12 months
  • Zhang, Cai, Song, Dong, Hou, Lv: "Normalization of ventral tegmental area structure following acupuncture in a rat model of heroin relapse." in: Neural regeneration research, Vol. 9, Issue 3, pp. 301-7, (2014) (PubMed).

  • Target
    GDNF
    Alternative Name
    GDNF (GDNF Products)
    Synonyms
    gdnf, GDNF, AI385739, gdnfa, hfb1-gdnf, ATF1, ATF2, HFB1-GDNF, HSCR3, glial cell derived neurotrophic factor, glial cell derived neurotrophic factor a, glial cell line derived neurotrophic factor, glial cell derived neurotrophic factor L homeolog, Gdnf, gdnfa, GDNF, gdnf.L
    Background

    Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.

    Subcellular location: Secreted

    Synonyms: ATF1, ATF2, HSCR3, HFB1-GDNF, Glial cell line-derived neurotrophic factor, hGDNF, Astrocyte-derived trophic factor, ATF, GDNF

    Gene ID
    2668
    UniProt
    P39905
    Pathways
    RTK Signaling, Synaptic Membrane, Tube Formation, Autophagy, Smooth Muscle Cell Migration
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