IL-5 antibody

Catalog No. ABIN116079

This anti-IL-5 antibody is a Rabbit Polyclonal antibody detecting IL-5 in WB, EIA, Func and IHC (p). Suitable for Human.

Quick Overview for IL-5 antibody (ABIN116079)

Target: IL-5 (IL5) (Interleukin 5 (IL5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-5 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-IL-5 Antibody

Specificity: This antibody reacts with Human Interleukin-5. Other species not tested.

Purification: Affinity Chromatography.

Immunogen: Highly pure (>98%) recombinant Human IL-5.

Application Details

Application Notes: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hIL-5(0.45 ng/mL), a concentration of 0.05-0.09 μg/mL of this antibody is required. ELISA: To detect hIL-5 by direct ELISA (using 100l/well antibody solution) aconcentration of at least 0.5 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2-0.4 ng/well of recombinant hIL-5. Western Blot: To detect hIL-5 by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hIL-5 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for IL-5

Target: IL-5 (IL5) (Interleukin 5 (IL5))

Alternative Name: Interleukin-5 / IL5

Background: Interleukin 5 (IL5) is a cytokine produced primarily by activated T lymphocytes. It exists as an antiparallel disulfide linked homodimeric glycoprotein with 115 amino acid residues in each chain. Known also as EDF (eosinophil differentiating factor), functions predominantly as an eosinophilopoietic factor. Analysis of its crystal structure reveals a novel two domain structure, with each domain showing significant homology to the cytokine fold in GMCSF, MCSF, IL2, IL4 and growth hormone. Human and mouse IL5 have 70 % amino acid sequence homology.Synonyms: B-cell differentiation factor I, Eosinophil differentiation factor, IL-5, T-cell replacing factor, TRF

Gene ID: 3567

NCBI Accession: NP_000870

UniProt: P05113

Pathways: JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour