IL-13 antibody

Catalog No. ABIN116107

This Rabbit Polyclonal antibody specifically detects IL-13 in WB, Func and EIA. It exhibits reactivity toward Human.

Quick Overview for IL-13 antibody (ABIN116107)

Target: IL-13 (IL13) (Interleukin 13 (IL13))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-13 antibody is un-conjugated

Application: Western Blotting (WB), Functional Studies (Func), Enzyme Immunoassay (EIA)

Product Details anti-IL-13 Antibody

Specificity: Human Interleukin-13.

Purification: Affinity Chromatography.

Immunogen: Highly pure (>98%) recombinant hIL-13.

Application Details

Application Notes: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhIL-13 (2.0 ng/mL), a concentration of 0.12-0.15 μg/mL of this antibody is required. Sandwih ELISA: To detect hIL-13 by Sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated anti-Human IL-13 (PP1034B1 or PP1034B2) as adetection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-13. Western Blot: To detect hIL-13 by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 4-8 °C for one month or at -20 °C for longer.

Target Details for IL-13

Target: IL-13 (IL13) (Interleukin 13 (IL13))

Alternative Name: Interleukin-13 / IL13

Background: Human IL13 was originally identified by differential screening of an anti CD28 activated human peripheral blood mononuclear cell cDNA library as an induction specific novel cytokine. It was also isolated from cDNA libraries of human T cell clones using the murine IL13 (P600) cDNA as a probe. Human IL13, a pleiotropic cytokine, is produced by activated Th0, Th1 like, Th2 like, and CD8 T cells. The gene for human IL13 maps to chromosome 5 and is closely linked to the genes for IL3, IL4, IL5, and GMCSF. IL13 inhibits proinflammatory cytokine production and stimulates antibody production. It induces proliferation in the human pre myeloid cell line TF1. IL13 has multiple effects on the differentiation and functions of monocytes and macrophages. It suppresses cytotoxic functions and induces changes in the morphology of human monocytes and in the phenotype of human monocytes and B cells by upregulating MHC class II expression. IL13 will also decrease the production of nitric oxide by activated murine macrophages, leading to impaired parasiticidal activity. Human and mouse interleukin 13 share approximately 58 % amino acid sequence identity. Although human and mouse IL13 are equally active on human cells, human IL13 is much less active than mouse IL13 on mouse cells. Human IL13 and human IL4 also share approximately 30 % sequence homology and have similar biological functions.Synonyms: IL-13, NC30

Gene ID: 3596

NCBI Accession: NP_002179

UniProt: P35225

Pathways: JAK-STAT Signaling, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport