FGF7 antibody

Catalog No. ABIN116127

Cited in 1 publication. The Rabbit Polyclonal anti-FGF7 antibody (ABIN116127) specifically detects FGF7 in WB, IHC (p), EIA and Func. The antibody is reactive with Human samples.

Quick Overview for FGF7 antibody (ABIN116127)

Target: FGF7 (Fibroblast Growth Factor 7 (FGF7))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FGF7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Functional Studies (Func)

Product Details anti-FGF7 Antibody

Specificity: This antibody reacts with Human Keratinocyte Growth Factor (KGF).

Purification: Affinity chromatography

Immunogen: Highly purified (>98%) recombinant FGF-7 (KGF)

Application Details

Application Notes: Neutralisation: To yield one-half maximal inhibition [ND50]of the biological activity of hKGF(25 ng/mL), a concentration of 0.56-0.85 μg/mL of this antibody has been used for previouslots. ELISA: To detect hKGF by direct ELISA (using 100 l/well antibody solution) a concentrationof at least 0.5g/mL of this antibody is required. This antigen affinity purified antibody, inconjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/wellof recombinant hKGF. Western blot: To detect hKGF by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hKGF is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

References for anti-FGF7 antibody (ABIN116127)

Lim, Poh, Riau, Beuerman, Tan, Mehta: "Biological and ultrastructural properties of acelagraft, a freeze-dried γ-irradiated human amniotic membrane." in: Archives of ophthalmology, Vol. 128, Issue 10, pp. 1303-10, (2010) (PubMed).

Target Details for FGF7

Target: FGF7 (Fibroblast Growth Factor 7 (FGF7))

Alternative Name: FGF7 / KGF

Background: KGF is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is a potent epithelial cell specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. Studies of mouse and rat homologs of this gene implicated roles in morphogenesis of epithelium, reepithelialization of wounds, hair development and early lung organogenesis.Synonyms: Fibroblast growth factor 7, HBGF7, Heparin-binding growth factor 7, Keratinocyte growth factor

Gene ID: 2252

NCBI Accession: NP_002000

UniProt: P21781

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway