Leptin antibody (Biotin)

Catalog No. ABIN116129

The Rabbit Polyclonal anti-Leptin antibody has been validated for WB and EIA. It is suitable to detect Leptin in samples from Human.

Quick Overview for Leptin antibody (Biotin) (ABIN116129)

Target: Leptin (LEP)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Leptin antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-Leptin Antibody (Biotin)

Specificity: This antibody reacts with Human Leptin. Other species not tested.

Purification: Affinity Chromatography

Immunogen: Highly pure (>98%) E.coli derived recombinant Human Leptin (Cat.-No PA094)

Application Details

Application Notes: ELISADirect: To detect hLeptin by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL of this antibody is required. This antibody, in conjunctionwith compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hLeptin. Sandwich: To detect hLeptin by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated antibody, inconjunction with Anti-Human Leptin (PP1041P) as a capture antibody, allows the detectionof at least 0.2 - 0.4 ng/well of recombinant hLeptin. Western Blot: To detect hLeptin by Western Blot analysis this antibody can be used at a concentration of0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant hLeptin is 1.5 - 3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Centrifuge vial prior to opening!

Buffer: PBS, pH 7.2 without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.

Target Details for Leptin

Target: Leptin (LEP)

Alternative Name: Leptin

Background: Leptin plays a critical role in the regulation of body weight by inhibiting food intake and stimulating energy expenditure. Defects in Leptin production cause severe hereditary obesity in rodents and humans. In addition to its effects on body weight, leptin has a variety of other functions, including the regulation of hematopoiesis, angiogenesis, wound healing, and the immune and inflammatory response. The Leptin gene is the human homolog of the gene (ob) mutant in the mouse 'obese' phenotype. Defects in the Leptin gene are the cause of profound obesity and type II diabetes.Synonyms: LEP, OB, OBS, Obese protein, Obesity factor

Gene ID: 3952

NCBI Accession: NP_000221

UniProt: P41159

Pathways: JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process