CCL8 antibody (Biotin)

Catalog No. ABIN116141

Product Details anti-CCL8 Antibody (Biotin)

Target: CCL8 (Chemokine (C-C Motif) Ligand 8 (CCL8))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL8 antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: Human Macrophage Chemotactic Protein-2 (MCP-2).

Purification: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant hMCP-2 (human MCP-2).

Application Details

Application Notes: ELISA: To detect hMCP-2 by direct ELISA (using 100 l/well antibody solution) this antibodycan be used at a concentration of 0.15 - 0.30 μg/mL. Used in conjunction with compatiblesecondary reagents, allows the detection of at least 0.2 ng/well of recombinant hMCP-2. Western Blot: To detect hMCP-2 by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hMCP-2 is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Storage: -20 °C

Storage Comment: Store the antibody in aliquots at -20 °C.

Target Details for CCL8

Target: CCL8 (Chemokine (C-C Motif) Ligand 8 (CCL8))

Alternative Name: MCP2 / CCL8 (CCL8 Products)

Synonyms: HC14 antibody, MCP-2 antibody, MCP2 antibody, SCYA10 antibody, SCYA8 antibody, 1810063B20Rik antibody, AB023418 antibody, Mcp2 antibody, Scya8 antibody, C-C motif chemokine ligand 8 antibody, chemokine (C-C motif) ligand 8 antibody, C-C motif chemokine 8 antibody, monocyte chemoattractant protein-2 precursor antibody, CCL8 antibody, Ccl8 antibody, LOC101119572 antibody, MCP-2 antibody

Background: MCP 2 (Monocyte Chemoattractant Protein 2) is a chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. MCP 2 is important to inflammatory host responses, and is found in the highest concentration in the small intestine and peripheral blood cells. This protein is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. By recruiting leukocytes to sites of inflammation this cytokine may contribute to tumor-associated leukocyte infiltration and to the antiviral state against HIV infection.Synonyms: C-C motif chemokine 8, CCL-8, HC14, MCP-2, Monocyte Chemotactic Protein-2, Monocyte chemoattractant protein 2, SCYA10, SCYA8, Small-inducible cytokine A8

Gene ID: 6355

NCBI Accession: NP_005614

UniProt: P80075