M-CSF/CSF1 antibody

Catalog No. ABIN116155

This Rabbit Polyclonal antibody specifically detects M-CSF/CSF1 in WB, IHC (p), EIA and Func. It exhibits reactivity toward Human.

Quick Overview for M-CSF/CSF1 antibody (ABIN116155)

Target: M-CSF/CSF1 (CSF1) (Colony Stimulating Factor 1 (Macrophage) (CSF1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This M-CSF/CSF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Functional Studies (Func)

Product Details anti-M-CSF/CSF1 Antibody

Specificity: Recognizes Human Macrophage Colony Stimulating Factor (M-CSF).

Purification: Affinity Chromatography

Immunogen: Highly pure (>98%) E.coli derived recombinant Human M-CSF (hM-CSF)

Application Details

Application Notes: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhM-CSF (7.0 ng/mL), a concentration of 0.02-0.03 μg/mL of this antibody is required. ELISA: (Indirect): To detect hM-CSF by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant hM-CSF. (Sandwich): To detect hM-CSF by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti-Human M-CSF (PP1048B) as a detectionantibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hM-CSF. Western Blot: To detect hM-CSF by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hM-CSF is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.

Target Details for M-CSF/CSF1

Target: M-CSF/CSF1 (CSF1) (Colony Stimulating Factor 1 (Macrophage) (CSF1))

Alternative Name: Macrophage Colony Stimulating Factor (M-CSF)

Background: Synonyms: CSF-1, CSF1, MCSF, Macrophage colony-stimulating factor 1

Gene ID: 1435

NCBI Accession: NP_000748

UniProt: P09603

Pathways: RTK Signaling