Oncostatin M antibody (Biotin)

Catalog No. ABIN116201

The Rabbit Polyclonal anti-Oncostatin M antibody is suitable to detect Oncostatin M in samples from Human. It has been validated for WB and EIA.

Quick Overview for Oncostatin M antibody (Biotin) (ABIN116201)

Target: Oncostatin M (OSM)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Oncostatin M antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-Oncostatin M Antibody (Biotin)

Specificity: Human Oncostatin M

Purification: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant hOncostatin M (human Oncostatin M).

Application Details

Application Notes: ELISA: To detect hOncostatin M by direct ELISA (using 100 l/well antibody solution) thisantibody can be used at a concentration of 0.15 - 0.30 μg/mL. Used in conjunction withcompatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinanthOncostatin M. Western Blot: To detect hOncostatin M by Western Blot analysis this antibody can be usedat a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant hOncostatin M is 1.5 - 3.0 ng/lane, undereither reducing or non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for Oncostatin M

Target: Oncostatin M (OSM)

Alternative Name: Oncostatin-M

Background: Synonyms: OSM

Gene ID: 5008

NCBI Accession: NP_065391

UniProt: P13725

Pathways: JAK-STAT Signaling, Negative Regulation of Hormone Secretion